WAK1_ARATH » Wall-associated receptor kinase 1

WAK1_ARATH » Wall-associated receptor kinase 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
WAK1_ARATH » Wall-associated receptor kinase 1 »
Hydrophobic Thickness 32.8 ± 2.0 Å
Tilt Angle 0 ± 2°
ΔGtransfer -48.5 kcal/mol
ΔGfold -23.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 330-356 (325-360)
Pathways none
PDB none
OPM none
Complexes none
Interactions

P2C70, Complex: WAK1:P2C70

Domains

AA: 29-129, Wall-associated receptor kinase galacturonan-binding

AA: 282-327, PDBID: 1NT0, Subunit A, Seq Identity:36%, Calcium-binding EGF domain

AA: 410-679, PDBID: 2NRU, Subunit B, Seq Identity:43%, Protein tyrosine kinase

UniProt annotation for WAK1_ARATH » Wall-associated receptor kinase 1
FUNCTION: Serine/threonine-protein kinase that may function as a signaling receptor of extracellular matrix component. Binding to pectin may have significance in the control of cell expansion, morphogenesis and development. Required during plant"s response to pathogen infection and in plant defense against heavy metal toxicity. Phosphorylates the oxygen-evolving enhancer protein 2 (OEE2) in an GRP-3-dependent manner.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

SUBUNIT: Interacts with the glycine-rich proteins GRP3 and GRP3S, and the type 2C protein phosphatase KAPP. Component of a 500 kDa complex, composed of WAK1, GRP3 and KAPP. Interacts with the oxygen-evolving enhancer protein 2 (OEE2).

TISSUE SPECIFICITY: Predominantly expressed in green tissues such as stems and leaves. Detected at organ junctions.

DEVELOPMENTAL STAGE: Expressed in shoot and root apical meristems, and in expanding leaves and sepals.

INDUCTION: Induced by salicylic acid (SA) or INA, methyl jasmonate, ethylene, wounding and pathogen infection. Accumulated in roots after Aluminum exposure. Up-regulated by GRP3.

MISCELLANEOUS: Binding to polygalacturonic acid (PGA) multimers is calcium-dependent.

UniProt features for WAK1_ARATH » Wall-associated receptor kinase 1
SIGNAL 1 24 Potential.
CHAIN 25 735 Wall-associated receptor kinase 1.
DOMAIN 234 281 EGF-like 1.
DOMAIN 282 328 EGF-like 2; calcium-binding (Potential).
DOMAIN 409 692 Protein kinase.
REGION 67 254 Polygalacturonic acid-binding.
ACT_SITE 534 534 Proton acceptor (By similarity).
DISULFID 238 253 By similarity.
DISULFID 247 264 By similarity.
DISULFID 266 280 By similarity.
DISULFID 286 303 By similarity.
DISULFID 297 312 By similarity.
DISULFID 314 327 By similarity.
Amino Acid Sequence for WAK1_ARATH » Wall-associated receptor kinase 1
MKVQEGLFLV AIFFSLACTQ LVKGQHQPGE NCQNKCGNIT IEYPFGISSG CYYPGNESFS ITCKEDRPHV LSDIEVANFN HSGQLQVLLN RSSTCYDEQG KKTEEDSSFT LENLSLSANN KLTAVGCNAL SLLDTFGMQN YSTACLSLCD SPPEADGECN GRGCCRVDVS APLDSYTFET TSGRIKHMTS FHDFSPCTYA FLVEDDKFNF SSTEDLLNLR NVMRFPVLLD WSVGNQTCEQ VGSTSICGGN STCLDSTPRN GYICRCNEGF DGNPYLSAGC QDVNECTTSS TIHRHNCSDP KTCRNKVGGF YCKCQSGYRL DTTTMSCKRK EFAWTTILLV TTIGFLVILL GVACIQQRMK HLKDTKLREQ FFEQNGGGML TQRLSGAGPS NVDVKIFTED GMKKATNGYA ESRILGQGGQ GTVYKGILPD NSIVAIKKAR LGDSSQVEQF INEVLVLSQI NHRNVVKLLG CCLETEVPLL VYEFITNGTL FDHLHGSMID SSLTWEHRLK IAIEVAGTLA YLHSSASIPI IHRDIKTANI LLDVNLTAKV ADFGASRLIP MDKEELETMV QGTLGYLDPE YYNTGLLNEK SDVYSFGVVL MELLSGQKAL CFKRPQSSKH LVSYFATATK ENRLDEIIGG EVMNEDNLKE IQEAARIAAE CTRLMGEERP RMKEVAAKLE ALRVEKTKHK WSDQYPEENE HLIGGHILSA QGETSSSIGY DSIKNVAILD IETGR