VSR1_ARATH » Vacuolar-sorting receptor 1

VSR1_ARATH » Vacuolar-sorting receptor 1
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Topology in Vesicle membrane
Topologyvesicle lumenal side
cytoplasmic side
VSR1_ARATH » Vacuolar-sorting receptor 1 » AtVSR1; BP80-like protein b;AtBP80b; Epidermal growth factor receptor-like protein 1;AtELP; AtELP1; Spot 3 protein;
Hydrophobic Thickness 24.8 ± 2.8 Å
Tilt Angle 2 ± 1°
ΔGtransfer -20.4 kcal/mol
ΔGfold -16.5 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 566-585 (566-592)
Pathways none
PDB 4tjv (20-182), 4tjx (A=20-182)
OPM none
Complexes none
Interactions

BETL2, Complex: VSR1:BETL2

PAM74, Complex: VSR1:PAM74

SYP21, Complex: SYP21:VSR1

UBC34, Complex: VSR1:UBC34

VTI11, Complex: VTI11:VSR1

WAK3, Complex: VSR1:WAK3

Domains

AA: 56-159, PDBID: 4TJV, Subunit A, Seq Identity:100%, PA domain

UniProt annotation for VSR1_ARATH » Vacuolar-sorting receptor 1
FUNCTION: Vacuolar-sorting receptor (VSR) involved in clathrin- coated vesicles sorting from Golgi apparatus to vacuoles. Required for the sorting of 12S globulin, 2S albumin and maybe other seed storage proteins to protein storage vacuoles (PSVs) in seeds. May also be implicated in targeting N-terminal propeptide containing proteins to lytic vacuoles.

SUBUNIT: Interacts with the N-terminal propeptide of ALEU and the C-terminal part of the 12S globulin CRA1 in a calcium-dependent manner. Binds to EPSIN1.

TISSUE SPECIFICITY: Mostly expressed in roots and dividing cells (at protein level). Also expressed in seeds, seedlings, leaves, stems, flowers, siliques and pollen.

INDUCTION: Slightly induced by water deficit.

DOMAIN: The tyrosine-based internalization signal may be involved in trafficking at the trans Golgi network (TGN).

UniProt features for VSR1_ARATH » Vacuolar-sorting receptor 1
SIGNAL 1 22 Potential.
CHAIN 23 623 Vacuolar-sorting receptor 1.
DOMAIN 51 163 PA.
DOMAIN 411 461 EGF-like 1.
DOMAIN 464 511 EGF-like 2.
DOMAIN 512 554 EGF-like 3; calcium-binding (Potential).
MOTIF 606 609 Tyrosine-based internalization motif.
DISULFID 415 433 By similarity.
DISULFID 422 442 By similarity.
DISULFID 444 460 By similarity.
DISULFID 468 488 By similarity.
DISULFID 475 496 By similarity.
DISULFID 498 510 By similarity.
DISULFID 540 553 By similarity.
Amino Acid Sequence for VSR1_ARATH » Vacuolar-sorting receptor 1
MKLGLFTLSF LLILNLAMGR FVVEKNNLKV TSPDSIKGIY ECAIGNFGVP QYGGTLVGTV VYPKSNQKAC KSYSDFDISF KSKPGRLPTF VLIDRGDCYF TLKAWIAQQA GAAAILVADS KAEPLITMDT PEEDKSDADY LQNITIPSAL ITKTLGDSIK SALSGGDMVN MKLDWTESVP HPDERVEYEL WTNSNDECGK KCDTQIEFLK NFKGAAQILE KGGHTQFTPH YITWYCPEAF TLSKQCKSQC INHGRYCAPD PEQDFTKGYD GKDVVVQNLR QACVYRVMND TGKPWVWWDY VTDFAIRCPM KEKKYTKECA DGIIKSLGID LKKVDKCIGD PEADVENPVL KAEQESQIGK GSRGDVTILP TLVVNNRQYR GKLEKGAVLK AMCSGFQEST EPAICLTEDL ETNECLENNG GCWQDKAANI TACRDTFRGR LCECPTVQGV KFVGDGYTHC KASGALHCGI NNGGCWRESR GGFTYSACVD DHSKDCKCPL GFKGDGVKNC EDVDECKEKT VCQCPECKCK NTWGSYECSC SNGLLYMREH DTCIGSGKVG TTKLSWSFLW ILIIGVGVAG LSGYAVYKYR IRSYMDAEIR GIMAQYMPLE SQPPNTSGHH MDI