VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10

VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10
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Topology in Golgi apparatus membrane
TopologyGolgi lumenal side
cytoplasmic side
VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10 » Carboxypeptidase Y receptor;CPY receptor; Carboxypeptidase Y-deficient protein 1;Sortilin VPS10;Vacuolar carboxypeptidase sorting receptor VPS10;Vacuolar protein sorting-associated protein 10;Vacuolar protein-targeting protein 1;
Hydrophobic Thickness 34.8 ± 1.7 Å
Tilt Angle 8 ± 7°
ΔGtransfer -31.7 kcal/mol
ΔGfold -26.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 1393-1415 (1393-1420)
Pathways none
PDB none
OPM none
Complexes none
Interactions

ANP1, Complex: VPS10:ANP1

ATG27, Complex: VPS10:ATG27, PubMed

ERG5, Complex: ERG5:VPS10

HOC1, Complex: HOC1:VPS10

KEX1, Complex: VPS10:KEX1, PubMed

KEX2, Complex: VPS10:KEX2, PubMed

MNN11, Complex: MNN11:VPS10

MRL1, Complex: VPS10:MRL1, PubMed

OSTB, Complex: OSTB:VPS10

PMP2, Complex: VPS10:PMP2, PubMed

PSD1, Complex: PSD1:VPS10

TLG2, Complex: VPS10:TLG2, PubMed

VAM3, Complex: VAM3:VPS10

VTH1, Complex: VPS10:VTH1

VTH2, Complex: VPS10:VTH2

VTI1, Complex: VPS10:VTI1, PubMed

YN94, Complex: PYR1:ACT:VATB:BZZ1:HXKA:GAL1:ATPA:ATPB:HSP42:HSP60:TBA1:TBB:RPN1:TBA3:LAS17:SLA1:HSP77:VPS10:MYO4:RV167:VRP1:ADT3:YN94:HXT6, PubMed

Domains

AA: 533-544, PDBID: 2W20, Subunit B, Seq Identity:57%, BNR/Asp-box repeat

AA: 559-716, PDBID: 3WSX, Subunit A, Seq Identity:24%, Sortilin, neurotensin receptor 3, C-terminal

AA: 1210-1378, PDBID: 1WGO, Subunit A, Seq Identity:26%, Sortilin, neurotensin receptor 3, C-terminal

UniProt annotation for VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10
FUNCTION: Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.

DOMAIN: The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.

MISCELLANEOUS: Present with 7210 molecules/cell in log phase SD medium.

UniProt features for VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10
SIGNAL 1 21 Potential.
CHAIN 22 1579 Vacuolar protein sorting/targeting protein VPS10.
REPEAT 57 68 BNR 1.
REPEAT 101 112 BNR 2.
REPEAT 179 190 BNR 3.
REPEAT 415 426 BNR 4.
REPEAT 487 498 BNR 5.
REPEAT 533 544 BNR 6.
REPEAT 764 775 BNR 7.
REPEAT 861 872 BNR 8.
REPEAT 1143 1154 BNR 9.
REPEAT 1184 1195 BNR 10.
Amino Acid Sequence for VPS10_YEAST » Vacuolar protein sorting/targeting protein VPS10
MILLHFVYSL WALLLIPLTN AEEFTPKVTK TIAQDSFDIL SFDDSNTLIR KQDTSVTISF DDGETWEKVE GIEGEITWIY IDPFNRHDRA VATAMNGSYL YITNDQGKSW ERITLPDSGE SISPRECYIE THPLNKNYFL AKCNYCEKTE VNNDNEENSG DEEGQFEIFN ITRCTDKVFA SNDGGKSFSE IKSSLERNEN SPISISDCGF AKTSKDSDLE SSDTSIICLF QNMQLIMDEF SSPYTESKLV LTTDWGKSLK EFDQFKDKVV NGYRILKSHM VVLTQGDRYN DMSSMDVWVS NDLSNFKMAY MPTQLRHSMQ GEIYEDAMGR IILPMSRERS DQEEDKGIVS EILISDSQGL KFSPIPWTAN EVFGYINFYQ PTYLKGTMIA SLYPLSRRRN RKGKAKGVKS KGVTKISVDN GLTWTMLKVV DPDNADSFDC DITDFENCSL QNMFYTREGS TPTAGILMTT GIVGDGSVFD WGDQRTFISR DGGLTWKLAF DFPCLYAVGD YGNVIVAIPY NADEDDDPQS EFYYSLDQGK TWTEYQLETT IYPNEVMNTT PDGSGAKFIL NGFTLAHMDG TTNFIYAIDF STAFNDKTCE ENDFEDWNLA EGKCVNGVKY KIRRRKQDAQ CLVKKVFEDL QLFETACDKC TEADYECAFE FVRDATGKCV PDYNLIVLSD VCDKTKKKTV PVKPLQLVKG DKCKKPMTVK SVDISCEGVP KKGTNDKEIV VTENKFDFKI QFYQYFDTVT DESLLMINSR GEAYISHDGG QTIKRFDSNG ETIIEVVFNP YYNSSAYLFG SKGSIFSTHD RGYSFMTAKL PEARQLGMPL DFNAKAQDTF IYYGGKNCES ILSPECHAVA YLTNDGGETF TEMLDNAIHC EFAGSLFKYP SNEDMVMCQV KEKSSQTRSL VSSTDFFQDD KNTVFENIIG YLSTGGYIIV AVPHENNELR AYVTIDGTEF AEAKFPYDED VGKQEAFTIL ESEKGSIFLH LATNLVPGRD FGNLLKSNSN GTSFVTLEHA VNRNTFGYVD FEKIQGLEGI ILTNIVSNSD KVAENKEDKQ LKTKITFNEG SDWNFLKPPK RDSEGKKFSC SSKSLDECSL HLHGYTERKD IRDTYSSGSA LGMMFGVGNV GPNLLPYKEC STFFTTDGGE TWAEVKKTPH QWEYGDHGGI LVLVPENSET DSISYSTDFG KTWKDYKFCA DKVLVKDITT VPRDSALRFL LFGEAADIGG SSFRTYTIDF RNIFERQCDF DITGKESADY KYSPLGSKSN CLFGHQTEFL RKTDENCFIG NIPLSEFSRN IKNCSCTRQD FECDYNFYKA NDGTCKLVKG LSPANAADVC KKEPDLIEYF ESSGYRKIPL STCEGGLKLD APSSPHACPG KEKEFKEKYS VSAGPFAFIF ISILLIIFFA AWFVYDRGIR RNGGFARFGE IRLGDDGLIE NNNTDRVVNN IVKSGFYVFS NIGSLLQHTK TNIAHAISKI RGRFGNRTGP SYSSLIHDQF LDEADDLLAG HDEDANDLSS FMDQGSNFEI EEDDVPTLEE EHTSYTDQPT TTDVPDTLPE GNEENIDRPD STAPSNENQ