VAMP8_HUMAN » Vesicle-associated membrane protein 8

VAMP8_HUMAN » Vesicle-associated membrane protein 8
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Topology in Vesicle membrane
Topologyvesicle lumenal side
cytoplasmic side
VAMP8_HUMAN » Vesicle-associated membrane protein 8 » VAMP-8; Endobrevin;EDB;
Hydrophobic Thickness 32.4 ± 5.8 Å
Tilt Angle 27 ± 4°
ΔGtransfer -31.1 kcal/mol
ΔGfold -25.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology In
TM Segments 75-99 (70-99)
Pathways

Membrane Trafficking (Reactome)

SNARE interactions in vesicular transport (KEGG)

PDB 4wy4 (A=11-74)
OPM 4wy4
Complexes

SNP29:STX17:VAMP8_HUMAN

STX7:VTI1B:VAMP8:STX8_MOUSE

Interactions

ITA4, Complex: ITA4:VAMP8, PubMed

STX17, Complex: SNP29:STX17:VAMP8, PDBID: 4WY4

STX1A, Complex: STX1A:VAMP8, PubMed

STX1B, Complex: VAMP8:STX1B, PubMed

STX2, Complex: VAMP8:STX2, PubMed

STX3, Complex: STX3:VAMP8, PubMed

STX4, Complex: VAPA:VAMP8:STX4:VAPB, PubMed

STX6, Complex: STX6:VAMP8, PubMed

STX7, Complex: STX7:STX8:VAMP8:VTI1B, PDBID: 1gl2

STX8, Complex: STX7:STX8:VAMP8:VTI1B, PDBID: 1gl2

VAMP2, Complex: VAMP8:VAMP2, PubMed

VAMP3, Complex: VAMP3:VAMP8, PubMed

VCAM1, Complex: VCAM1:VAMP8, PubMed

VTI1A, Complex: VTI1A:VAMP8, PubMed

VTI1B, Complex: STX7:STX8:VAMP8:VTI1B, PDBID: 1gl2

Domains

AA: 9-97, PDBID: 4WY4, Subunit A, Seq Identity:100%, Synaptobrevin

UniProt annotation for VAMP8_HUMAN » Vesicle-associated membrane protein 8
FUNCTION: SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

SUBUNIT: Forms a SNARE complex composed of VAMP8, SNAP29 and STX17 involved in fusion of autophagosome with lysosome. Binds tightly to multiple syntaxins. Found in a number of SNARE complexes with NAPA, SNAP23, SNAP25, STX1A, STX4, STX7, STX8 and VTI1B.

TISSUE SPECIFICITY: Platelets.

UniProt features for VAMP8_HUMAN » Vesicle-associated membrane protein 8
CHAIN 1 100 Vesicle-associated membrane protein 8.
DOMAIN 12 72 v-SNARE coiled-coil homology.
SITE 33 33 Interaction with STX8 (By similarity).
Amino Acid Sequence for VAMP8_HUMAN » Vesicle-associated membrane protein 8
MEEASEGGGN DRVRNLQSEV EGVKNIMTQN VERILARGEN LEHLRNKTED LEATSEHFKT TSQKVARKFW WKNVKMIVLI CVIVFIIILF IVLFATGAFS