UBX2_YEAST » UBX domain-containing protein 2

UBX2_YEAST » UBX domain-containing protein 2
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
UBX2_YEAST » UBX domain-containing protein 2 » Secretion lowering protein 1;
Hydrophobic Thickness 27.4 ± 1.5 Å
Tilt Angle 3 ± 2°
ΔGtransfer -13.2 kcal/mol
ΔGfold -6.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 151-169 (151-170)
Pathways

Protein processing in endoplasmic reticulum (KEGG)

PDB none
OPM none
Complexes none
Interactions

CP51, Complex: CP51:UBX2, PubMed

CWH41, Complex: CWH41:UBX2

EMP47, Complex: HRD1:UBX2:OS9:EMP47:USA1:CBPY:HRD3:CDC48:GRP78, PubMed

ERG2, Complex: ERG2:UBX2

ERG5, Complex: ERG5:UBX2

HRD3, Complex: CDC48:UBX2:NPL4:UFD1:HRD1:USA1:HRD3:OS9:DER1, PubMed

HRD3, Complex: GRP78:CDC48:UBX1:UBX2:NPL4:HRD1:USA1:HRD3:OS9:UBX7:DFM1, PubMed

HRD3, Complex: UBX2:HRD3, PubMed

IRE1, Complex: IRE1:UBX2

MGA2, Complex: UBX2:MGA2

MMM1, Complex: UBX2:MMM1

SC6B2, Complex: SC6B2:UBX2

SEC66, Complex: SEC66:UBX2

SLG1, Complex: SLG1:UBX2

TMEDA, Complex: TMEDA:UBX2

VPS64, Complex: UBX2:VPS64

YN8B, Complex: YN8B:UBX2

YNS1, Complex: YNS1:UBX2

Domains

AA: 18-62, PDBID: 4F1I, Subunit A, Seq Identity:30%, UBA-like domain

AA: 426-570, PDBID: 1WJ4, Subunit A, Seq Identity:15%, UBX domain

UniProt annotation for UBX2_YEAST » UBX domain-containing protein 2
FUNCTION: Integral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the UFD1-NPL4-CDC48/p97 (UNC) AAA ATPase complex and targeted to the proteasome.

SUBUNIT: Component of the DOA10 complex which contains SSM4/DOA10, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the membrane integral E3 ligase SSM4/DOA10 and the heterotrimeric UFD1-NPL4-CDC48/p97 (UNC) ATPase complex recuited by UBX2/SEL1. Component of the HRD1 complex which contains HRD1, HRD3, USA1, DER1, YOS9, KAR2, CDC48, NPL4, UFD1 AND UBX2/SEL1. The complex is composed of the core membrane complex, consisting of the E3 ligase HRD1 and its cofactors HRD3, DER1 and USA1, the substrate recruiting factors KAR2 and YOS9, and the heterotrimeric UFD1- NPL4-CDC48/p97 (UNC) ATPase complex recruited by UBX2/SEL1. Interacts with CDC48.

MISCELLANEOUS: Present with 12600 molecules/cell in log phase SD medium.

UniProt features for UBX2_YEAST » UBX domain-containing protein 2
CHAIN 1 584 UBX domain-containing protein 2.
DOMAIN 426 570 UBX.
Amino Acid Sequence for UBX2_YEAST » UBX domain-containing protein 2
MPVVNHEDSE FHLSHTEEDK LNEFQVITNF PPEDLPDVVR LLRNHGWQLE PALSRYFDGE WKGEPDQMGE PTQTSTPMAE TLVPPALGPR PLLFTASLPV VRPLPANFRN DFRTIGLNGR SNTVWSMFES FSYDGNPFLF ILLLIPRIIN RLSATIFTFF CTLLSLHSIS GGGNSGKPKI SKVPKAPTRE THIPLAEILG DTKDKDAFCE LKSFKPDISF NEALRIAKEE FKFMLLILVG DTYDTDTDTV DVNSKLLLEK ILLNKKTLQY LRKIDNDLII YLKCVHELEP WLVARQLGVR NTPEIFLIAN VANKASHSET LPSQRLSILG KLKVNSLNRF LQSLTNVVEK YTPELVVNKT EMHELRMSRE IKKLQEDAYK KSLEMDRIKA IEKEKSLKHA QDLKLNSTAR QLKWLKACID EIQPFETTGK QATLQFRTSS GKRFVKKFPS MTTLYQIYQS IGCHIYLAVY SSDPAEWSNA LQDKIRQLSA DDDMLCFKEG QLETATATTI EELGHIINNE LTSFDLERGK LEFDFELVSP FPKYTVHPNE HMSVDQVPQL WPNGSLLVEA LDEEDEEDEE NEEQ