UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32

UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32 » Ubiquitin carrier protein 32;
Hydrophobic Thickness 32.2 ± 3.0 Å
Tilt Angle 11 ± 11°
ΔGtransfer -24.5 kcal/mol
ΔGfold -16.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 274-295 (270-298)
Pathways

Protein processing in endoplasmic reticulum (KEGG)

Ubiquitin mediated proteolysis (KEGG)

PDB none
OPM none
Complexes none
Interactions

ACCR2, Complex: ACCR2:UBC32

ATL16, Complex: ATL16:UBC32

ATL4, Complex: ATL4:UBC32

ATL78, Complex: ATL78:UBC32

ATL79, Complex: ATL79:UBC32

BAME2, Complex: BAME2:UBC32

CRK19, Complex: CRK19:UBC32

CRK2, Complex: CRK2:UBC32

CRK31, Complex: CRK31:UBC32

ERL1, Complex: ERL1:UBC32

ERL1, Complex: ERL1:UBC32

FEI2, Complex: FEI2:UBC32

GSO1, Complex: GSO1:UBC32

LRK42, Complex: LRK42:UBC32

LRK55, Complex: LRK55:UBC32

LRK63, Complex: LRK63:UBC32

LYK5, Complex: LYK5:UBC32

MRH1, Complex: MRH1:UBC32

PERK5, Complex: PERK5:UBC32

RLK90, Complex: RLK90:UBC32

SD113, Complex: SD113:UBC32

SIRK, Complex: SIRK:UBC32

SRF6, Complex: SRF6:UBC32

WAK3, Complex: WAK3:UBC32

Y1105, Complex: Y1105:UBC32

Y1133, Complex: Y1133:UBC32

Y1661, Complex: Y1661:UBC32

Y1756, Complex: Y1756:UBC32

Y1765, Complex: Y1765:UBC32

Y2165, Complex: Y2165:UBC32

Y2182, Complex: Y2182:UBC32

Y2393, Complex: Y2393:UBC32

Y3868, Complex: Y3868:UBC32

Y4294, Complex: Y4294:UBC32

Y4361, Complex: Y4361:UBC32

Y4372, Complex: Y4372:UBC32

Y5169, Complex: Y5169:UBC32

Y5188, Complex: Y5188:UBC32

Y5241, Complex: Y5241:UBC32

Y5371, Complex: Y5371:UBC32

Y5389, Complex: Y5389:UBC32

Y5659, Complex: Y5659:UBC32

Y5838, Complex: Y5838:UBC32

Y5977, Complex: Y5977:UBC32

Domains

AA: 15-134, PDBID: 2F4W, Subunit A, Seq Identity:45%, Ubiquitin-conjugating enzyme

UniProt annotation for UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32
FUNCTION: Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.

CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

UniProt features for UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32
CHAIN 1 309 Ubiquitin-conjugating enzyme E2 32.
ACT_SITE 93 93 Glycyl thioester intermediate (By similarity).
Amino Acid Sequence for UBC32_ARATH » Ubiquitin-conjugating enzyme E2 32
MADERYNRKN PAVKRILQEV KEMQANPSDD FMSLPLEENI FEWQFAIRGP GDTEFEGGIY HGRIQLPADY PFKPPSFMLL TPNGRFETNT KICLSISNYH PEHWQPSWSV RTALVALIAF MPTSPNGALG SVDYPKDERR TLAIKSRETP PKYGSPERQK IIDEIHQYIL SKATVVPKPL PLECSQAPSI VSEAHSQVEP QEAITVVEER SIATTDTIVD DQIIEETAEA VNTAASVVPA AAPLPAVEVV VKASVSGEQR MARRAAQKPV DDRLFTWAAV GLTIAIMVLL LKKFIKSNGY STGFMDDQS