TPSN_HUMAN » Tapasin

TPSN_HUMAN » Tapasin
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
TPSN_HUMAN » Tapasin » TPN; TPSN; NGS-17;TAP-associated protein;TAP-binding protein;
Hydrophobic Thickness 30.0 ± 3.4 Å
Tilt Angle 27 ± 0°
ΔGtransfer -25.0 kcal/mol
ΔGfold -15.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC, Reactome
Topology Out
TM Segments 414-435 (411-445)
Pathways

Antigen processing and presentation (KEGG)

Immune System (Reactome)

PDB 3f8u (B/D=1-401)
OPM none
Complexes none
Interactions none
Domains

AA: 292-389, PDBID: 3F8U, Subunit D, Seq Identity:100%, Immunoglobulin C1-set domain

UniProt annotation for TPSN_HUMAN » Tapasin
FUNCTION: Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).

SUBUNIT: Heterodimer with PDIA3; disulfide-linked. Interacts with TAP1 and is thus a subunit of the TAP complex, also known as the peptide loading complex (PLC). Interaction with TAP1 is TAP2- independent and is required for efficient peptide-TAP interaction. Obligatory mediator for the interaction between newly assembled MHC class I molecules, calreticulin, PDIA3 and TAP. Up to 4 MHC class I/tapasin complexes bind to 1 TAP.

TISSUE SPECIFICITY: Neutrophils, mostly in fully differentiated cells.

DOMAIN: The N-terminus is required for efficient association with MHC class I molecule and for a stable interaction between MHC I and calreticulin. Binding to TAP is mediated by the C-terminal region.

UniProt features for TPSN_HUMAN » Tapasin
SIGNAL 1 20
CHAIN 21 448 Tapasin.
DOMAIN 292 399 Ig-like C1-type.
SITE 428 428 May be involved in interaction with TAP.
DISULFID 27 91
DISULFID 115 115 Interchain (with C-57 in PDIA3).
DISULFID 315 382
Amino Acid Sequence for TPSN_HUMAN » Tapasin
MKSLSLLLAV ALGLATAVSA GPAVIECWFV EDASGKGLAK RPGALLLRQG PGEPPPRPDL DPELYLSVHD PAGALQAAFR RYPRGAPAPH CEMSRFVPLP ASAKWASGLT PAQNCPRALD GAWLMVSISS PVLSLSSLLR PQPEPQQEPV LITMATVVLT VLTHTPAPRV RLGQDALLDL SFAYMPPTSE AASSLAPGPP PFGLEWRRQH LGKGHLLLAA TPGLNGQMPA AQEGAVAFAA WDDDEPWGPW TGNGTFWLPR VQPFQEGTYL ATIHLPYLQG QVTLELAVYK PPKVSLMPAT LARAAPGEAP PELLCLVSHF YPSGGLEVEW ELRGGPGGRS QKAEGQRWLS ALRHHSDGSV SLSGHLQPPP VTTEQHGARY ACRIHHPSLP ASGRSAEVTL EVAGLSGPSL EDSVGLFLSA FLLLGLFKAL GWAAVYLSTC KDSKKKAE