TPBG_HUMAN » Trophoblast glycoprotein

TPBG_HUMAN » Trophoblast glycoprotein
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
TPBG_HUMAN » Trophoblast glycoprotein » 5T4 oncofetal antigen;5T4 oncofetal trophoblast glycoprotein;5T4 oncotrophoblast glycoprotein; M6P1;
Hydrophobic Thickness 34.0 ± 1.1 Å
Tilt Angle 2 ± 1°
ΔGtransfer -27.9 kcal/mol
ΔGfold -23.5 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Out
TM Segments 354-376 (351-379)
Pathways none
PDB 4cnc (60-345), 4cnm (60-345)
OPM none
Complexes none
Interactions

NICA, Complex: NICA:TPBG, PubMed

Domains

AA: 61-90, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat N-terminal domain

AA: 91-154, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat

AA: 118-168, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat

AA: 210-270, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat

AA: 239-294, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat

AA: 320-345, PDBID: 4CNC, Subunit B, Seq Identity:100%, Leucine rich repeat C-terminal domain

UniProt annotation for TPBG_HUMAN » Trophoblast glycoprotein
FUNCTION: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a- dependent LRP6 internalization.

TISSUE SPECIFICITY: Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

DOMAIN: The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway.

MISCELLANEOUS: Antigen 5T4 is overexpressed by a wide spectrum of cancers, including colorectal, ovarian and gastric, but with a limited normal tissue expression. Could be used for tumor immunotherapy.

MISCELLANEOUS: Reduction of TPBG levels by siRNA significantly enhanced the beta-catenin/TCF transcription-based reporter pBAR activation in response to Wnt stimulation.

UniProt features for TPBG_HUMAN » Trophoblast glycoprotein
SIGNAL 1 31 Potential.
CHAIN 32 420 Trophoblast glycoprotein.
DOMAIN 53 91 LRRNT.
REPEAT 92 113 LRR 1.
REPEAT 119 140 LRR 2.
REPEAT 143 164 LRR 3.
REPEAT 211 232 LRR 4.
REPEAT 235 256 LRR 5.
REPEAT 259 280 LRR 6.
DOMAIN 294 346 LRRCT.
Amino Acid Sequence for TPBG_HUMAN » Trophoblast glycoprotein
MPGGCSRGPA AGDGRLRLAR LALVLLGWVS SSSPTSSASS FSSSAPFLAS AVSAQPPLPD QCPALCECSE AARTVKCVNR NLTEVPTDLP AYVRNLFLTG NQLAVLPAGA FARRPPLAEL AALNLSGSRL DEVRAGAFEH LPSLRQLDLS HNPLADLSPF AFSGSNASVS APSPLVELIL NHIVPPEDER QNRSFEGMVV AALLAGRALQ GLRRLELASN HFLYLPRDVL AQLPSLRHLD LSNNSLVSLT YVSFRNLTHL ESLHLEDNAL KVLHNGTLAE LQGLPHIRVF LDNNPWVCDC HMADMVTWLK ETEVVQGKDR LTCAYPEKMR NRVLLELNSA DLDCDPILPP SLQTSYVFLG IVLALIGAIF LLVLYLNRKG IKKWMHNIRD ACRDHMEGYH YRYEINADPR LTNLSSNSDV