TOLA_ECOLI » Protein TolA

TOLA_ECOLI » Protein TolA
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Topology in Bacterial Gram-negative inner membrane
Topologyextracellular side
cytoplasmic side
TOLA_ECOLI » Protein TolA »
Hydrophobic Thickness 30.4 ± 1.4 Å
Tilt Angle 29 ± 0°
ΔGtransfer -18.5 kcal/mol
ΔGfold -17.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 10-34 (9-34)
Pathways none
PDB 1tol (295-421), 3qdp (302-421), 1s62 (325-421), 3qdr (A=302-421)
OPM none
Complexes none
Interactions

TOLR, Complex: TOLA:TOLR

Domains

AA: 325-419, PDBID: 1S62, Subunit A, Seq Identity:100%, TolA C-terminal

UniProt annotation for TOLA_ECOLI » Protein TolA
FUNCTION: Involved in the TonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA.

SUBUNIT: Interacts, via domain II, with porins OmpC, PhoE and LamB.

UniProt features for TOLA_ECOLI » Protein TolA
CHAIN 1 421 Protein TolA.
REPEAT 224 229 1.
REPEAT 230 234 2.
REPEAT 235 240 3.
REPEAT 241 245 4.
REPEAT 246 250 5.
REPEAT 251 255 6.
REPEAT 256 260 7.
REPEAT 261 266 8.
REPEAT 267 271 9.
REPEAT 272 277 10.
REPEAT 278 282 11.
REPEAT 283 287 12.
REPEAT 288 292 13.
REGION 48 310 Domain II (alpha-helical).
REGION 224 292 13 X tandem repeats of [EDA]-K(1,2)- A(2,4).
REGION 311 421 Domain III (functional).
DISULFID 363 388
Amino Acid Sequence for TOLA_ECOLI » Protein TolA
MSKATEQNDK LKRAIIISAV LHVILFAALI WSSFDENIEA SAGGGGGSSI DAVMVDSGAV VEQYKRMQSQ ESSAKRSDEQ RKMKEQQAAE ELREKQAAEQ ERLKQLEKER LAAQEQKKQA EEAAKQAELK QKQAEEAAAK AAADAKAKAE ADAKAAEEAA KKAAADAKKK AEAEAAKAAA EAQKKAEAAA AALKKKAEAA EAAAAEARKK AATEAAEKAK AEAEKKAAAE KAAADKKAAA EKAAADKKAA EKAAAEKAAA DKKAAAEKAA ADKKAAAAKA AAEKAAAAKA AAEADDIFGE LSSGKNAPKT GGGAKGNNAS PAGSGNTKNN GASGADINNY AGQIKSAIES KFYDASSYAG KTCTLRIKLA PDGMLLDIKP EGGDPALCQA ALAAAKLAKI PKPPSQAVYE VFKNAPLDFK P