TOC34_ARATH » Translocase of chloroplast 34, chloroplastic

TOC34_ARATH » Translocase of chloroplast 34, chloroplastic
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Topology in Chloroplast outer membrane
Topologyintermembrane space
cytoplasmic side
TOC34_ARATH » Translocase of chloroplast 34, chloroplastic » AtToc34; 34 kDa chloroplast outer envelope protein;GTP-binding protein OEP34;Plastid protein import 3;
Hydrophobic Thickness 31.2 ± 0.8 Å
Tilt Angle 0 ± 0°
ΔGtransfer -24.4 kcal/mol
ΔGfold -4.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 266-289 (262-291)
Pathways none
PDB none
OPM none
Complexes

TOC34:TOC34_PEA

Interactions

OEP61, Complex: TOC34:OEP61

TC120, Complex: TC120:TOC34, PubMed

TC132, Complex: TC132:TOC34, PubMed

TOC34, Complex: Homodimer of chloroplast translocase 34, PDBID: 3BB1

Domains

AA: 39-235, PDBID: 2J3E, Subunit A, Seq Identity:68%, AIG1 family

UniProt annotation for TOC34_ARATH » Translocase of chloroplast 34, chloroplastic
FUNCTION: GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast.

SUBUNIT: Homodimer, heterodimer with TOC33, and monomer. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Interacts at least with TOC75.

TISSUE SPECIFICITY: Mostly expressed in roots and flowers, and, to a lower extent, in seedlings, stems, and leaves.

DEVELOPMENTAL STAGE: Expressed at a relatively uniform, low level at most stages of development. Observed in cotyledons and hypocotyls of young seedling. Expressed in apical meristems. Found in the whole roots. Expressed in middle-aged leaves. In flowers, mostly localized in meristems, but is also present in all organs.

INDUCTION: Induced in light but repressed in darkness.

UniProt features for TOC34_ARATH » Translocase of chloroplast 34, chloroplastic
CHAIN 1 313 Translocase of chloroplast 34, chloroplastic.
REGION 67 70 Homodimerization (By similarity).
REGION 127 132 Homodimerization (By similarity).
Amino Acid Sequence for TOC34_ARATH » Translocase of chloroplast 34, chloroplastic
MAALQTLREW IGIQQFPPAT QSKLLEILGK YKEEDVSSLT VLVMGKGGVG KSSTVNSVIG EKAAAVSTFQ SEGLRPTLVS RTRSGFTLNI IDTPGLIEGG YVNDQAINII KRFLLNMTID VLLYVDRLDV YRVDDLDRQV VGAITDAFGK EIWKKSALVL THAQFSPPDG LNYNHFVSKR SNALLKVIQT GAQLKKQDLQ GFSIPVILVE NSGRCHKNES DEKILPCGTS WIPNLFNKIT EISFNGNKAI HVDKKLVEGP NPNERGKKLI PLMFAFQYLL VMKPLVRAIK SDVSRESKPA WELRDSGLAS RRS