TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1

TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1
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Topology in Vesicle membrane
Topologyvesicle lumenal side
cytoplasmic side
TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1 » Syntaxin TLG1;
Hydrophobic Thickness 31.2 ± 2.7 Å
Tilt Angle 1 ± 0°
ΔGtransfer -23.9 kcal/mol
ΔGfold -19.2 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 204-224 (202-224)
Pathways

SNARE interactions in vesicular transport (KEGG)

PDB 2c5j (A/B=1-95), 2c5i (T=1-101), 2c5k (T=1-95)
OPM none
Complexes

TLG1:TLG1_YEAST

Interactions

AXL2, Complex: AXL2:TLG1, PubMed

GOSR1, Complex: TLG1:GOSR1

MKAR, Complex: MKAR:TLG1, PubMed

NYV1, Complex: TLG1:NYV1, PubMed

PEP12, Complex: TLG1:PEP12, PubMed

SEC22, Complex: TLG1:SEC22

SED5, Complex: SED5:TLG1, PubMed

SNC1, Complex: TLG1:SNC1, PubMed

SNC2, Complex: TLG1:SNC2, PubMed

TLG1, Complex: Homodimer of t-SNARE affecting a late Golgi compartment protein 1, PDBID: 2C5J

TLG2, Complex: GRP78:HSP72:SEC17:VPS45:YKT6:VTI1:TLG2:TLG1, PubMed

VAM3, Complex: TLG1:VAM3, PubMed

VTI1, Complex: VTI1:TLG1, PubMed

Domains

AA: 6-88, PDBID: 2c5i, Subunit T, Seq Identity:100%, Syntaxin 6, N-terminal

AA: 137-199, SNARE domain

UniProt annotation for TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1
FUNCTION: SNARE protein (of Qc type) involved in membrane fusion probably in retrograde traffic of cytosolic double-membrane vesicles derived from both, early and possibly late endosomes/PVC (prevacuolar compartment) back to the trans-Golgi network (TGN or late Golgi). It has been reported to function both as a (target membrane) t-SNARE and as a (vesicle) v-SNARE. Upon vesicle tethering to the target membrane, which requires additional proteins, a SNARE-pin is formed. This is a very stable 4 parallel alpha-helical coil bundle consisting of 4 SNARE domains (usually one of each type: Qa, Qb, Qc, and R), of which at least one is anchored in the opposite membrane. The formation of the SNARE-pin is believed to bring the two membranes in close proximity and to provide the energy to drive membrane fusion. Through its interaction with the VFT (or GARP) complex, it may also contribute to vesicle recognition specificity and tethering. Regulation of SNARE-pin formation also seems to depend on the phosphorylation state of the protein, phosphorylation by TPK1 causing inhibition and dephosphorylation by SIT4 activation.

SUBUNIT: Interacts in a SNARE-pin with the SNAREs TLG2 (Qa), VTI1 (Qb), and SNC1 or SNC2 (R). Interacts with VPS51 of the VFT (or GARP) complex.

UniProt features for TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1
CHAIN 1 224 T-SNARE affecting a late Golgi compartment protein 1.
DOMAIN 132 194 t-SNARE coiled-coil homology.
REGION 1 106 Interaction with VPS51.
COILED 35 101 Potential.
Amino Acid Sequence for TLG1_YEAST » T-SNARE affecting a late Golgi compartment protein 1
MNNSEDPFQQ VVKDTKEQLN RINNYITRHN TAGDDDQEEE IQDILKDVEE TIVDLDRSII VMKRDENEDV SGREAQVKNI KQQLDALKLR FDRRIQESTQ TTIPLEETVE NSTLNTSMAE NNDGGMSNPF QEQMLREQDV HLDGIHKTMQ NLHIQAQTMG DELENQGQLL DNMDEGMDGV VNKLARGRRQ LEWVYEKNKE KYDDCCIGLL IVVLIVLLVL AFIA