TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50

TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50
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Topology in Mitochondrial inner membrane
Topologyintermembrane space
mitochondrial matrix side
TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50 »
Hydrophobic Thickness 27.0 ± 2.4 Å
Tilt Angle 24 ± 12°
ΔGtransfer -11.0 kcal/mol
ΔGfold -9.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 112-132 (105-133)
Pathways

Mitochondrial Protein Import (yeast) (Reactome)

PDB none
OPM none
Complexes none
Interactions

FCJ1, Complex: TIM50:FCJ1, PubMed

HLJ1, Complex: TIM50:HLJ1, PubMed

TIM14, Complex: TIM44:TIM14:TIM50:TIM23:TIM16:TIM17, PubMed

TIM21, Complex: TIM50:TIM21, PubMed

TOM22, Complex: TIM50:TOM22, PubMed

TOM70, Complex: TOM70:TIM50, PubMed

UCRI, Complex: UCRI:TIM50

Domains

AA: 192-339, PDBID: 4QQF, Subunit D, Seq Identity:100%, NLI interacting factor-like phosphatase

UniProt annotation for TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50
FUNCTION: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.

SUBUNIT: Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with preproteins in transit.

DOMAIN: The mitochondrial intermembrane region is required for generation but not stabilization of the TIM23 complex.

DOMAIN: The FCP1 homology domain does not contain the canonical D- x-D-x-[TV] active site, suggesting that it probably does not display phosphatase activity.

MISCELLANEOUS: Present with 2400 molecules/cell in log phase SD medium.

UniProt features for TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50
TRANSIT 1 43 Mitochondrion (Probable).
CHAIN 44 476 Mitochondrial import inner membrane translocase subunit TIM50.
DOMAIN 187 330 FCP1 homology.
Amino Acid Sequence for TIM50_YEAST » Mitochondrial import inner membrane translocase subunit TIM50
MLSILRNSVR LNSRALRVVP SAANTLTSVQ ASRRLLTSYS SFLQKETKDD KPKSILTDDM LFKAGVDVDE KGQGKNEETS GEGGEDKNEP SSKSEKSRRK RQTSTDIKRE KYANWFYIFS LSALTGTAIY MARDWEPQES EELKKDIDNG YTLSLMYKRF KARFNSMFTY FQEPPFPDLL PPPPPPPYQR PLTLVITLED FLVHSEWSQK HGWRTAKRPG ADYFLGYLSQ YYEIVLFSSN YMMYSDKIAE KLDPIHAFVS YNLFKEHCVY KDGVHIKDLS KLNRDLSKVI IIDTDPNSYK LQPENAIPME PWNGEADDKL VRLIPFLEYL ATQQTKDVRP ILNSFEDKKN LAEEFDHRVK KLKDKFYGDH KSGGNWAMTA LGLGNSLGGS TKFPLDLIHE EGQKNYLMFM KMIEEEKEKI RIQQEQMGGQ TFTLKDYVEG NLPSPEEQMK IQLEKQKEVD ALFEEEKKKK KIAESK