TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21

TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21
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Topology in Mitochondrial inner membrane
Topologyintermembrane space
mitochondrial matrix side
TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21 »
Hydrophobic Thickness 31.8 ± 4.2 Å
Tilt Angle 36 ± 26°
ΔGtransfer -24.6 kcal/mol
ΔGfold -15.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 71-96 (71-97)
Pathways

Mitochondrial Protein Import (yeast) (Reactome)

PDB 2ciu (103-225), 2mf7 (103-225)
OPM none
Complexes none
Interactions

FCJ1, Complex: TIM21:FCJ1, PubMed

MIA40, Complex: DYR:CYB2:MIA40:TIM21:TIM17, PubMed

MIA40, Complex: MIA40:TIM21, PubMed

NCPR, Complex: COM1:VPS15:PRP28:BAR1:GYL1:SLK19:HNT1:RV167:RV161:PCL10:NCPR:RRF2M:TIM21:AIM3:GYP5:COA4:KIN2, PubMed

QCR8, Complex: QCR8:TIM21

TIM14, Complex: TIM44:TIM14:TIM50:TIM21:TIM23:TIM17, PubMed

TIM50, Complex: TIM50:TIM21, PubMed

TOM22, Complex: TIM21:TOM22, PubMed

UCRI, Complex: UCRI:TIM21

Domains

AA: 67-219, PDBID: 2CIU, Subunit A, Seq Identity:100%, TIM21

UniProt annotation for TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21
FUNCTION: Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to keep the TOM and the TIM23 complexes in close contact. At some point, it is released from the TOM23 complex to allow protein translocation into the mitochondrial matrix. In the complex, it acts as an antagonist of TIM50 by reducing preprotein accumulation at the TOM23 complex and promotes dissociation of the PAM complex from the TIM23 complex.

SUBUNIT: Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. Interacts directly with TIM23. Interacts with TOM22 component of the TOM complex. Released from the TOM23 complex by the PAM complex, leading to protein translocation into the mitochondrial matrix.

MISCELLANEOUS: Present with 3770 molecules/cell in log phase SD medium.

UniProt features for TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21
TRANSIT 1 70 Mitochondrion (Potential).
CHAIN 71 239 Mitochondrial import inner membrane translocase subunit TIM21.
Amino Acid Sequence for TIM21_YEAST » Mitochondrial import inner membrane translocase subunit TIM21
MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD