TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14

TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14
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Topology in Mitochondrial inner membrane
Topologyintermembrane space
mitochondrial matrix side
TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14 » Presequence translocated-associated motor subunit PAM18;
Hydrophobic Thickness 27.6 ± 2.6 Å
Tilt Angle 1 ± 0°
ΔGtransfer -30.0 kcal/mol
ΔGfold -8.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 65-84 (63-88)
Pathways

Mitochondrial Protein Import (yeast) (Reactome)

PDB 2guz (A/C...=99-168)
OPM none
Complexes none
Interactions

GEM1, Complex: TIM14:GEM1

MDM38, Complex: MDM38:TIM14

MIA40, Complex: TIM14:MIA40

PHB2, Complex: TIM14:PHB2

TIM21, Complex: TIM44:TIM14:TIM50:TIM21:TIM23:TIM17, PubMed

TIM50, Complex: TIM14:TIM50, PubMed

TIM50, Complex: TIM44:TIM14:TIM50:TIM23:TIM16:TIM17, PubMed

TOM22, Complex: TIM14:TOM22, PubMed

TOM70, Complex: TOM70:TIM14

Domains

AA: 112-165, PDBID: 2guz, Subunit C, Seq Identity:100%, DnaJ domain

UniProt annotation for TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14
FUNCTION: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).

SUBUNIT: Homodimer and heterodimer with PAM16/TIM16. Homodimerization may not be relevant in vivo, while heterodimerization is essential for activity regulation of mtHSP70. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18/TIM14. Interacts directly with mtHsp70. Interacts directly with TIM17 subunit of the TIM23 complex.

DOMAIN: The J domain is essential for co-chaperone activity and mediates the heterodimerization with the J-like domain of PAM16.

UniProt features for TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14
CHAIN 1 168 Mitochondrial import inner membrane translocase subunit TIM14.
DOMAIN 112 168 J.
Amino Acid Sequence for TIM14_YEAST » Mitochondrial import inner membrane translocase subunit TIM14
MSSQSNTGNS IEAPQLPIPG QTNGSANVTV DGAGVNVGIQ NGSQGQKTGM DLYFDQALNY MGEHPVITGF GAFLTLYFTA GAYKSISKGL NGGKSTTAFL KGGFDPKMNS KEALQILNLT ENTLTKKKLK EVHRKIMLAN HPDKGGSPFL ATKINEAKDF LEKRGISK