TIC40_ARATH » Protein TIC 40, chloroplastic

TIC40_ARATH » Protein TIC 40, chloroplastic
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Topology in Chloroplast inner membrane
Topologyintermembrane space
chloroplast stroma
TIC40_ARATH » Protein TIC 40, chloroplastic » Protein PIGMENT DEFECTIVE EMBRYO 120;Translocon at the inner envelope membrane of chloroplasts 40;AtTIC40;
Hydrophobic Thickness 26.8 ± 1.2 Å
Tilt Angle 9 ± 2°
ΔGtransfer -15.6 kcal/mol
ΔGfold -6.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 108-126 (107-130)
Pathways none
PDB 2lnm (386-447)
OPM none
Complexes none
Interactions none
Domains none
UniProt annotation for TIC40_ARATH » Protein TIC 40, chloroplastic
FUNCTION: Involved in protein precursor import into chloroplasts. Part of the motor complex consisting of a co-chaperone (TIC40) and a chaperone (HSP93) associated with the import channel (TIC110). Causes the release of bound transit peptides from TIC110 and stimulates ATP hydrolysis by HSP93. Involved in reinsertion of proteins from the chloroplast stroma into the inner membrane.

SUBUNIT: Part of the Tic complex. Interacts with HSP93, TIC110 and LTD.

TISSUE SPECIFICITY: Expressed in seedlings, flowers, leaves, stems and roots.

DEVELOPMENTAL STAGE: Expressed throughout development.

DOMAIN: The C-terminal half (89-105) of the Ser/Pro-rich region and the transmembrane domain are necessary and sufficient for membrane integration.

DOMAIN: The TPR region (238-373) interacts with TIC110.

DOMAIN: The STI1 2 domain (386-425) has a stimulatory effect on HSP93 ATP hydrolysis.

MISCELLANEOUS: Inserts into the inner envelope membrane from the stroma after import from the cytoplasm. The transit peptide undergoes a two-step processing. The initial cleavage to generate the intermediate found in the stroma is mediated by the stromal processing peptidase (SPP) while the final processing step by a signal peptidase I-type (SPase I), possibly PLSP1, requires association with the inner membrane.

UniProt features for TIC40_ARATH » Protein TIC 40, chloroplastic
TRANSIT 1 43 Chloroplast (Potential).
TRANSIT 44 76 Chloroplast; inner membrane (Potential).
CHAIN 77 447 Protein TIC 40, chloroplastic.
DOMAIN 310 344 STI1 1.
DOMAIN 386 425 STI1 2.
Amino Acid Sequence for TIC40_ARATH » Protein TIC 40, chloroplastic
MENLTLVSCS ASSPKLLIGC NFTSSLKNPT GFSRRTPNIV LRCSKISASA QSQSPSSRPE NTGEIVVVKQ RSKAFASIFS SSRDQQTTSV ASPSVPVPPP SSSTIGSPLF WIGVGVGLSA LFSYVTSNLK KYAMQTAMKT MMNQMNTQNS QFNNSGFPSG SPFPFPFPPQ TSPASSPFQS QSQSSGATVD VTATKVETPP STKPKPTPAK DIEVDKPSVV LEASKEKKEE KNYAFEDISP EETTKESPFS NYAEVSETNS PKETRLFEDV LQNGAGPANG ATASEVFQSL GGGKGGPGLS VEALEKMMED PTVQKMVYPY LPEEMRNPET FKWMLKNPQY RQQLQDMLNN MSGSGEWDKR MTDTLKNFDL NSPEVKQQFN QIGLTPEEVI SKIMENPDVA MAFQNPRVQA ALMECSENPM NIMKYQNDKE VMDVFNKISQ LFPGMTG