TC132_ARATH » Translocase of chloroplast 132, chloroplastic

TC132_ARATH » Translocase of chloroplast 132, chloroplastic
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Topology in Chloroplast outer membrane
Topologyintermembrane space
cytoplasmic side
TC132_ARATH » Translocase of chloroplast 132, chloroplastic » AtToc132; 132 kDa chloroplast outer envelope protein;
Hydrophobic Thickness 24.4 ± 2.2 Å
Tilt Angle 2 ± 0°
ΔGtransfer -28.7 kcal/mol
ΔGfold -3.1 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 1177-1198 (1176-1199)
Pathways none
PDB none
OPM none
Complexes none
Interactions

TC120, Complex: TC132:TC120, PubMed

TOC34, Complex: TC132:TOC34, PubMed

Domains

AA: 575-712, PDBID: 2J3E, Subunit A, Seq Identity:33%, AIG1 family

AA: 931-1197, Translocase of chloroplast 159/132, membrane anchor domain

UniProt annotation for TC132_ARATH » Translocase of chloroplast 132, chloroplastic
FUNCTION: GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast.

SUBUNIT: Homodimer (By similarity). Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG).

TISSUE SPECIFICITY: Expressed in seedlings, leaves, flowers, and roots.

INDUCTION: By light conditions.

UniProt features for TC132_ARATH » Translocase of chloroplast 132, chloroplastic
CHAIN 1 1206 Translocase of chloroplast 132, chloroplastic.
REGION 603 606 Homodimerization (By similarity).
REGION 666 671 Homodimerization (By similarity).
COILED 13 33 Potential.
Amino Acid Sequence for TC132_ARATH » Translocase of chloroplast 132, chloroplastic
MGDGTEFVVR SDREDKKLAE DRISDEQVVK NELVRSDEVR DDNEDEVFEE AIGSENDEQE EEEDPKRELF ESDDLPLVET LKSSMVEHEV EDFEEAVGDL DETSSNEGGV KDFTAVGESH GAGEAEFDVL ATKMNGDKGE GGGGGSYDKV ESSLDVVDTT ENATSTNTNG SNLAAEHVGI ENGKTHSFLG NGIASPKNKE VVAEVIPKDD GIEEPWNDGI EVDNWEERVD GIQTEQEVEE GEGTTENQFE KRTEEEVVEG EGTSKNLFEK QTEQDVVEGE GTSKDLFENG SVCMDSESEA ERNGETGAAY TSNIVTNASG DNEVSSAVTS SPLEESSSGE KGETEGDSTC LKPEQHLASS PHSYPESTEV HSNSGSPGVT SREHKPVQSA NGGHDVQSPQ PNKELEKQQS SRVHVDPEIT ENSHVETEPE VVSSVSPTES RSNPAALPPA RPAGLGRASP LLEPASRAPQ QSRVNGNGSH NQFQQAEDST TTEADEHDET REKLQLIRVK FLRLAHRLGQ TPHNVVVAQV LYRLGLAEQL RGRNGSRVGA FSFDRASAMA EQLEAAGQDP LDFSCTIMVL GKSGVGKSAT INSIFDEVKF CTDAFQMGTK RVQDVEGLVQ GIKVRVIDTP GLLPSWSDQA KNEKILNSVK AFIKKNPPDI VLYLDRLDMQ SRDSGDMPLL RTISDVFGPS IWFNAIVGLT HAASVPPDGP NGTASSYDMF VTQRSHVIQQ AIRQAAGDMR LMNPVSLVEN HSACRTNRAG QRVLPNGQVW KPHLLLLSFA SKILAEANAL LKLQDNIPGR PFAARSKAPP LPFLLSSLLQ SRPQPKLPEQ QYGDEEDEDD LEESSDSDEE SEYDQLPPFK SLTKAQMATL SKSQKKQYLD EMEYREKLLM KKQMKEERKR RKMFKKFAAE IKDLPDGYSE NVEEESGGPA SVPVPMPDLS LPASFDSDNP THRYRYLDSS NQWLVRPVLE THGWDHDIGY EGVNAERLFV VKEKIPISVS GQVTKDKKDA NVQLEMASSV KHGEGKSTSL GFDMQTVGKE LAYTLRSETR FNNFRRNKAA AGLSVTHLGD SVSAGLKVED KFIASKWFRI VMSGGAMTSR GDFAYGGTLE AQLRDKDYPL GRFLTTLGLS VMDWHGDLAI GGNIQSQVPI GRSSNLIARA NLNNRGAGQV SVRVNSSEQL QLAMVAIVPL FKKLLSYYYP QTQYGQ