TATA_ECOLI » Sec-independent protein translocase protein TatA

TATA_ECOLI » Sec-independent protein translocase protein TatA
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Topology in Bacterial Gram-negative inner membrane
Topologyextracellular side
cytoplasmic side
TATA_ECOLI » Sec-independent protein translocase protein TatA »
Hydrophobic Thickness 30.4 ± 2.5 Å
Tilt Angle 5 ± 4°
ΔGtransfer -22.2 kcal/mol
ΔGfold -17.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 4-25 (3-25)
Pathways

Bacterial secretion system (KEGG)

Protein export (KEGG)

PDB 2lzr (1-49), 2lzs (A/B...=1-49)
OPM 2lzr, 2lzs
Complexes

TATA:TATA_ECOLI

Interactions

TATA, Complex: Nonamer of Sec-independent protein translocase TatA, PDBID: 2LZS

TATB, Complex: TATA:TATB

Domains

AA: 4-83, PDBID: 2LZR, Subunit A, Seq Identity:100%, mttA/Hcf106 family

UniProt annotation for TATA_ECOLI » Sec-independent protein translocase protein TatA
FUNCTION: Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.

SUBUNIT: The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon. A complex containing only TatA and TatB has also been identified. It could be either an assembly intermediate or a disassembly intermediate generated during translocation activity. Each of TatA, TatB and TatC are able to interact in pairs without the third partner; TatA also forms homooligomers.

INDUCTION: Constitutively expressed. TatA is the most highly expressed of the tat genes.

MISCELLANEOUS: TatA forms a transmembrane channel with a lid structure on the cytoplasmic side. The number of TatA protomers can vary so that the channel can adopt different diameters to accommodate substrates of various sizes. This enables TatA to pack tightly around substrate and prevents ion leakage during transport.

MISCELLANEOUS: Absence of TatA from the TatBC complex leads to the instability of the complex and the specific breakdown of TatB.

UniProt features for TATA_ECOLI » Sec-independent protein translocase protein TatA
CHAIN 1 89 Sec-independent protein translocase protein TatA.
Amino Acid Sequence for TATA_ECOLI » Sec-independent protein translocase protein TatA
MGGISIWQLL IIAVIVVLLF GTKKLGSIGS DLGASIKGFK KAMSDDEPKQ DKTSQDADFT AKTIADKQAD TNQEQAKTED AKRHDKEQV