SPAST_DICDI » Spastin

SPAST_DICDI » Spastin
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Topology in Endosome membrane
Topologyendosome lumenal side
cytoplasmic side
SPAST_DICDI » Spastin »
Hydrophobic Thickness 31.2 ± 5.4 Å
Tilt Angle 28 ± 20°
ΔGtransfer -29.9 kcal/mol
ΔGfold -19.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 47-69 (43-74)
Pathways none
PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 169-230, PDBID: 1WFD, Subunit A, Seq Identity:26%, MIT (microtubule interacting and transport) domain

AA: 420-550, PDBID: 3VFD, Subunit A, Seq Identity:80%, ATPase family associated with various cellular activities (AAA)

AA: 609-653, PDBID: 3B9P, Subunit A, Seq Identity:55%, Vps4 C terminal oligomerisation domain

UniProt annotation for SPAST_DICDI » Spastin
FUNCTION: ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the microtubule organizing center following nucleation.

CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.

SUBUNIT: Homohexamer. The homohexamer is stabilized by ATP- binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules.

UniProt features for SPAST_DICDI » Spastin
CHAIN 1 655 Spastin.
DOMAIN 169 232 MIT.
Amino Acid Sequence for SPAST_DICDI » Spastin
MLFDLINSFL KNGINNSNNN NNNNNNKNNF YNSLEDDDYL LNNQTTKVSL YLYFFIFAFM FLVVDLIMLY YKHRENIESR ETDLSLKLNK MLIDFENDNK IKSSPTTSTT TTTITPTTTS SSQLRQPSTP KTTTKTINSP PSTPKSPPPL PSLESKLLYK DDIKQQLSLN EAKSQIDSAK QLDESLKYNS CIKLYIDGIE KLMALFSSYN SKEYRDYIDF YLKRAEYLKN ELKKGTNLKS ITNFNNFSKE YQINYNNKIL EQQQQQQQQS SSTYRNSLNL SSSKSNSTIN NRHSISSLSS LNSTTATTTT PSNTSTITSP GNKYGLQKSL SSTTLSLKKS SNSTNFQQPS PPSMVIPDIK GIDKSMVTLI MNEIMDRKNP VKWDDVVGLD KVKQSLMESV ILPNLRPDVF TGLRAPPKGL LLFGPPGNGK TMIAKAVAYE SKVTFFSISS SSLTSKYVGD GEKLVRALFA VATHFQPSII FIDEIDSLLT ERSSNESEAS RRLKTEILVQ FDGARTNGDE RVLVMGATNR PEDLDDAALR RLVKRIYVGL PELETRLQII QHLLVGQRHS LTKQQINSLA EVTQGYSGFD LAALCKDAAY EPIRRLGIGI KDLELNEISL ISFKDFANSL KQIRPSVTSQ SLKSFEKWNQ KFGTI