SERK1_ARATH » Somatic embryogenesis receptor kinase 1

SERK1_ARATH » Somatic embryogenesis receptor kinase 1
Magnify SERK1_ARATH » Somatic embryogenesis receptor kinase 1Enlarged view of image
3D view in GLMol or JMol

gray dot

Download Coordinates

gray dot

Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
SERK1_ARATH » Somatic embryogenesis receptor kinase 1 » AtSERK1; Somatic embryogenesis receptor-like kinase 1;
Hydrophobic Thickness 34.8 ± 2.2 Å
Tilt Angle 0 ± 0°
ΔGtransfer -38.6 kcal/mol
ΔGfold -17.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 240-262 (236-266)
Pathways none
PDB 4lsc (24-213), 4lsx (C/D=24-213)
OPM none
Complexes

SERK1:BRI1_ARATH

Interactions

BAK1, Complex: BAK1:SERK1

BRI1, Complex: SERK1:BRI1, PDBID: 4lsx, PubMed

CRK26, Complex: CRK26:SERK1

P2C70, Complex: SERK1:P2C70, PubMed

SERK2, Complex: SERK2:SERK1

Y5838, Complex: Y5838:SERK1, PubMed

Domains

AA: 26-66, PDBID: 4LSC, Subunit A, Seq Identity:100%, Leucine rich repeat N-terminal domain

AA: 166-191, PDBID: 4M7E, Subunit C, Seq Identity:69%, Leucine Rich Repeat

AA: 302-576, PDBID: 3TL8, Subunit A, Seq Identity:98%, Protein kinase domain

UniProt annotation for SERK1_ARATH » Somatic embryogenesis receptor kinase 1
FUNCTION: Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Phosphorylates BRI1 on "Ser- 887" and CDC48 on at least one threonine residue and on "Ser-41". Confers embryogenic competence. Acts redundantly with SERK2 as a control point for sporophytic development controlling male gametophyte production. Involved in the brassinolide signaling pathway.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

ENZYME REGULATION: Inhibited by manganese.

SUBUNIT: Monomer, homo- and heterodimer. Interacts with KAPP, CDC48A, GRF6 or GRF7, SERK2, BRI1 and SERK3/BAK1 to form the SERK1 signaling complex. Interacts with the EF-Tu receptor EFR and FLS2 in a specific ligand-induced manner.

TISSUE SPECIFICITY: Expressed in flowers, tapetum, developing microspores, all cells of the embryo sac, provascular strands and developing vascular bundles. Low expression in adult vascular tissue. Detected in root meristem.

DEVELOPMENTAL STAGE: Expressed during pollen development and megasporogenesis in the nucellus of developing ovules, in all cells of the embryo sac up to fertilization and in all cells of the developing embryo until the heart-shaped stage. Found in epidermal and vascular cells of the late torpedo and cotyledon stages embryos.

DOMAIN: The extracellular domain (26-234) is required for dimerization.

MISCELLANEOUS: Seems to be related with early development of tissues in general rather than with embryogenesis.

UniProt features for SERK1_ARATH » Somatic embryogenesis receptor kinase 1
SIGNAL 1 23 Potential.
CHAIN 24 625 Somatic embryogenesis receptor kinase 1.
REPEAT 94 117 LRR 1.
REPEAT 118 140 LRR 2.
REPEAT 142 165 LRR 3.
REPEAT 166 188 LRR 4.
DOMAIN 302 589 Protein kinase.
ACT_SITE 429 429 Proton acceptor (By similarity).
Amino Acid Sequence for SERK1_ARATH » Somatic embryogenesis receptor kinase 1
MESSYVVFIL LSLILLPNHS LWLASANLEG DALHTLRVTL VDPNNVLQSW DPTLVNPCTW FHVTCNNENS VIRVDLGNAE LSGHLVPELG VLKNLQYLEL YSNNITGPIP SNLGNLTNLV SLDLYLNSFS GPIPESLGKL SKLRFLRLNN NSLTGSIPMS LTNITTLQVL DLSNNRLSGS VPDNGSFSLF TPISFANNLD LCGPVTSHPC PGSPPFSPPP PFIQPPPVST PSGYGITGAI AGGVAAGAAL LFAAPAIAFA WWRRRKPLDI FFDVPAEEDP EVHLGQLKRF SLRELQVASD GFSNKNILGR GGFGKVYKGR LADGTLVAVK RLKEERTPGG ELQFQTEVEM ISMAVHRNLL RLRGFCMTPT ERLLVYPYMA NGSVASCLRE RPPSQPPLDW PTRKRIALGS ARGLSYLHDH CDPKIIHRDV KAANILLDEE FEAVVGDFGL AKLMDYKDTH VTTAVRGTIG HIAPEYLSTG KSSEKTDVFG YGIMLLELIT GQRAFDLARL ANDDDVMLLD WVKGLLKEKK LEMLVDPDLQ TNYEERELEQ VIQVALLCTQ GSPMERPKMS EVVRMLEGDG LAEKWDEWQK VEILREEIDL SPNPNSDWIL DSTYNLHAVE LSGPR