SEC12_YEAST » Guanine nucleotide-exchange factor SEC12

SEC12_YEAST » Guanine nucleotide-exchange factor SEC12
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
SEC12_YEAST » Guanine nucleotide-exchange factor SEC12 » Protein transport protein SEC12;
Hydrophobic Thickness 31.4 ± 3.2 Å
Tilt Angle 27 ± 4°
ΔGtransfer -21.2 kcal/mol
ΔGfold -14.4 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 352-373 (345-375)
Pathways none
PDB 4h5i (1-354), 4h5j (1-354)
OPM none
Complexes none
Interactions

HLJ1, Complex: HLJ1:SEC12

IRE1, Complex: IRE1:SEC12

NEM1, Complex: NEM1:SEC12

SED4, Complex: SEC12:SED4

Domains

AA: 300-337, PDBID: 1a0r, Subunit B, Seq Identity:32%, WD domain, G-beta repeat

UniProt annotation for SEC12_YEAST » Guanine nucleotide-exchange factor SEC12
FUNCTION: Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER. This function involves the cytoplasmic domain of the protein, which is thought to interact with the small GTP-binding protein SAR1. Required for autophagy.

MISCELLANEOUS: In the process of transport, SEC12 itself may migrate to the Golgi apparatus and function in subsequent transport events.

MISCELLANEOUS: Present with 6160 molecules/cell in log phase SD medium.

UniProt features for SEC12_YEAST » Guanine nucleotide-exchange factor SEC12
CHAIN 1 471 Guanine nucleotide-exchange factor SEC12.
REPEAT 259 300 WD 1.
REPEAT 302 341 WD 2.
Amino Acid Sequence for SEC12_YEAST » Guanine nucleotide-exchange factor SEC12
MKFVTASYNV GYPAYGAKFL NNDTLLVAGG GGEGNNGIPN KLTVLRVDPT KDTEKEQFHI LSEFALEDND DSPTAIDASK GIILVGCNEN STKITQGKGN KHLRKFKYDK VNDQLEFLTS VDFDASTNAD DYTKLVYISR EGTVAAIASS KVPAIMRIID PSDLTEKFEI ETRGEVKDLH FSTDGKVVAY ITGSSLEVIS TVTGSCIARK TDFDKNWSLS KINFIADDTV LIAASLKKGK GIVLTKISIK SGNTSVLRSK QVTNRFKGIT SMDVDMKGEL AVLASNDNSI ALVKLKDLSM SKIFKQAHSF AITEVTISPD STYVASVSAA NTIHIIKLPL NYANYTSMKQ KISKFFTNFI LIVLLSYILQ FSYKHNLHSM LFNYAKDNFL TKRDTISSPY VVDEDLHQTT LFGNHGTKTS VPSVDSIKVH GVHETSSVNG TEVLCTESNI INTGGAEFEI TNATFREIDD A