RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2

RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2
Magnify RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2Enlarged view of image
3D view in GLMol or JMol

gray dot

Download Coordinates

gray dot

Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2 » Protein TOADSTOOL 2;Receptor-like protein kinase 2;
Hydrophobic Thickness 31.2 ± 1.8 Å
Tilt Angle 6 ± 2°
ΔGtransfer -49.4 kcal/mol
ΔGfold -22.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 813-835 (809-836)
Pathways none
PDB none
OPM none
Complexes none
Interactions

BETL2, Complex: RPK2:BETL2

CLV1, Complex: CLV1:RPK2

PAM74, Complex: RPK2:PAM74

Domains

AA: 43-83, PDBID: 4J0M, Subunit B, Seq Identity:39%, Leucine rich repeat N-terminal domain

AA: 183-228, PDBID: 2Z63, Subunit A, Seq Identity:51%, Leucine rich repeat

AA: 263-285, PDBID: 4MN8, Subunit A, Seq Identity:58%, Leucine Rich Repeat

AA: 659-719, PDBID: 4M7E, Subunit C, Seq Identity:48%, Leucine rich repeat

AA: 874-1142, PDBID: 4OA2, Subunit A, Seq Identity:44%, Protein kinase domain

UniProt annotation for RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2
FUNCTION: Key regulator of anther development (e.g. lignification pattern), including tapetum degradation during pollen maturation (e.g. germination capacity). Together with RPK1, required for pattern formation along the radial axis (e.g. the apical embryonic domain cell types that generate cotyledon primordia), and the apical-basal axis (e.g. differentiation of the basal pole during early embryogenesis).

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

TISSUE SPECIFICITY: Expressed in root tips, flowers, leaf meristems, and the bottom parts of the surrounding young leaves and trichomes.

DEVELOPMENTAL STAGE: First detected in the central domain protodermal cells when cotyledon primordia become recognizable, at the early globular stage. Later observed throughout the central domain and basal domain of the embryo proper. After bolting, localized in axillary buds and premature anthers. Abundant in the tapetum of wild-type anthers during microspore maturation.

INDUCTION: Slightly by dehydration and low temperature.

UniProt features for RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2
SIGNAL 1 35 Potential.
CHAIN 36 1151 LRR receptor-like serine/threonine- protein kinase RPK2.
REPEAT 85 108 LRR 1.
REPEAT 143 167 LRR 2.
REPEAT 168 190 LRR 3.
REPEAT 192 215 LRR 4.
REPEAT 216 241 LRR 5.
REPEAT 261 285 LRR 6.
REPEAT 287 308 LRR 7.
REPEAT 309 333 LRR 8.
REPEAT 341 368 LRR 9.
REPEAT 387 410 LRR 10.
REPEAT 411 435 LRR 11.
REPEAT 436 459 LRR 12.
REPEAT 461 481 LRR 13.
REPEAT 542 565 LRR 14.
REPEAT 567 581 LRR 15.
REPEAT 585 608 LRR 16.
REPEAT 610 633 LRR 17.
REPEAT 634 657 LRR 18.
REPEAT 658 681 LRR 19.
REPEAT 682 706 LRR 20.
REPEAT 707 729 LRR 21.
REPEAT 731 752 LRR 22.
DOMAIN 874 1146 Protein kinase.
ACT_SITE 996 996 Proton acceptor (By similarity).
Amino Acid Sequence for RPK2_ARATH » LRR receptor-like serine/threonine-protein kinase RPK2
MTSLPSSVIK WRFFRRQMPS DVVFSLCLLC FASCLAGKIT VLADSDKSVL LRFKKTVSDP GSILASWVEE SEDYCSWFGV SCDSSSRVMA LNISGSGSSE ISRNRFTCGD IGKFPLYGFG VRRDCTGNHG ALAGNLPSVI MSLTGLRVLS LPFNSFSGEI PVGIWGMEKL EVLDLEGNLM TGSLPDQFTG LRNLRVMNLG FNRVSGEIPN SLQNLTKLEI LNLGGNKLNG TVPGFVGRFR VLHLPLNWLQ GSLPKDIGDS CGKLEHLDLS GNFLTGRIPE SLGKCAGLRS LLLYMNTLEE TIPLEFGSLQ KLEVLDVSRN TLSGPLPVEL GNCSSLSVLV LSNLYNVYED INSVRGEADL PPGADLTSMT EDFNFYQGGI PEEITRLPKL KILWVPRATL EGRFPGDWGS CQNLEMVNLG QNFFKGEIPV GLSKCKNLRL LDLSSNRLTG ELLKEISVPC MSVFDVGGNS LSGVIPDFLN NTTSHCPPVV YFDRFSIESY SDPSSVYLSF FTEKAQVGTS LIDLGSDGGP AVFHNFADNN FTGTLKSIPL AQERLGKRVS YIFSAGGNRL YGQFPGNLFD NCDELKAVYV NVSFNKLSGR IPQGLNNMCT SLKILDASVN QIFGPIPTSL GDLASLVALN LSWNQLQGQI PGSLGKKMAA LTYLSIANNN LTGQIPQSFG QLHSLDVLDL SSNHLSGGIP HDFVNLKNLT VLLLNNNNLS GPIPSGFATF AVFNVSSNNL SGPVPSTNGL TKCSTVSGNP YLRPCHVFSL TTPSSDSRDS TGDSITQDYA SSPVENAPSQ SPGKGGFNSL EIASIASASA IVSVLIALVI LFFYTRKWHP KSKIMATTKR EVTMFMDIGV PITFDNVVRA TGNFNASNLI GNGGFGATYK AEISQDVVVA IKRLSIGRFQ GVQQFHAEIK TLGRLRHPNL VTLIGYHASE TEMFLVYNYL PGGNLEKFIQ ERSTRDWRVL HKIALDIARA LAYLHDQCVP RVLHRDVKPS NILLDDDCNA YLSDFGLARL LGTSETHATT GVAGTFGYVA PEYAMTCRVS DKADVYSYGV VLLELLSDKK ALDPSFVSYG NGFNIVQWAC MLLRQGRAKE FFTAGLWDAG PHDDLVEVLH LAVVCTVDSL STRPTMKQVV RRLKQLQPPS C