ROBO1_HUMAN » Roundabout homolog 1

ROBO1_HUMAN » Roundabout homolog 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
ROBO1_HUMAN » Roundabout homolog 1 » Deleted in U twenty twenty;H-Robo-1;
Hydrophobic Thickness 30.8 ± 3.2 Å
Tilt Angle 0 ± 0°
ΔGtransfer -35.6 kcal/mol
ΔGfold -17.4 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Out
TM Segments 898-920 (897-925)
Pathways

Axon guidance (KEGG)

Developmental Biology (Reactome)

PDB 2eo9 (454-564), 2v9q (61-266), 2v9r (61-266), 4hlj (660-897), 3wih (A/B=777-873), 2v9t (A=61-166)
OPM none
Complexes none
Interactions

ROBO2, Complex: ROBO1:ROBO2, PubMed

ROBO4, Complex: ROBO1:ROBO4, PubMed

Domains

AA: 67-151, PDBID: 2V9Q, Subunit A, Seq Identity:100%, Immunoglobulin domain

AA: 170-258, PDBID: 2V9Q, Subunit A, Seq Identity:100%, Immunoglobulin I-set domain

AA: 262-347, PDBID: 2V9Q, Subunit A, Seq Identity:100%, Immunoglobulin I-set domain

AA: 350-432, PDBID: 1CS6, Subunit A, Seq Identity:31%, Immunoglobulin domain

AA: 454-529, PDBID: 2EO9, Subunit A, Seq Identity:100%, Immunoglobulin domain

AA: 562-646, PDBID: 1UEM, Subunit A, Seq Identity:55%, Fibronectin type III domain

AA: 678-758, PDBID: 4HLJ, Subunit A, Seq Identity:100%, Fibronectin type III domain

AA: 777-864, PDBID: 4HLJ, Subunit A, Seq Identity:100%, Fibronectin type III domain

UniProt annotation for ROBO1_HUMAN » Roundabout homolog 1
FUNCTION: Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.

SUBUNIT: Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding. Interacts with SLIT1 and SLIT2.

TISSUE SPECIFICITY: Widely expressed, with exception of kidney.

MISCELLANEOUS: Maps within a region of overlapping homozygous deletions characterized in both small cell lung cancer cell lines (SCLC) and in a breast cancer cell line. The promoter region of ROBO1 shows complete hypermethylation of CpG sites in the BT-20 breast tumor cell lines, some primary invasive breast carcinomasa and some primary clear cell renal cell carcinomas (CC-RCC).

UniProt features for ROBO1_HUMAN » Roundabout homolog 1
SIGNAL 1 25 Potential.
CHAIN 26 1651 Roundabout homolog 1.
DOMAIN 68 164 Ig-like C2-type 1.
DOMAIN 170 257 Ig-like C2-type 2.
DOMAIN 262 346 Ig-like C2-type 3.
DOMAIN 351 446 Ig-like C2-type 4.
DOMAIN 455 541 Ig-like C2-type 5.
DOMAIN 561 646 Fibronectin type-III 1.
DOMAIN 673 763 Fibronectin type-III 2.
DOMAIN 775 864 Fibronectin type-III 3.
DISULFID 89 147
DISULFID 191 240 Potential.
DISULFID 283 330 Potential.
DISULFID 372 428 Potential.
DISULFID 476 525 Potential.
Amino Acid Sequence for ROBO1_HUMAN » Roundabout homolog 1
MKWKHVPFLV MISLLSLSPN HLFLAQLIPD PEDVERGNDH GTPIPTSDND DNSLGYTGSR LRQEDFPPRI VEHPSDLIVS KGEPATLNCK AEGRPTPTIE WYKGGERVET DKDDPRSHRM LLPSGSLFFL RIVHGRKSRP DEGVYVCVAR NYLGEAVSHN ASLEVAILRD DFRQNPSDVM VAVGEPAVME CQPPRGHPEP TISWKKDGSP LDDKDERITI RGGKLMITYT RKSDAGKYVC VGTNMVGERE SEVAELTVLE RPSFVKRPSN LAVTVDDSAE FKCEARGDPV PTVRWRKDDG ELPKSRYEIR DDHTLKIRKV TAGDMGSYTC VAENMVGKAE ASATLTVQEP PHFVVKPRDQ VVALGRTVTF QCEATGNPQP AIFWRREGSQ NLLFSYQPPQ SSSRFSVSQT GDLTITNVQR SDVGYYICQT LNVAGSIITK AYLEVTDVIA DRPPPVIRQG PVNQTVAVDG TFVLSCVATG SPVPTILWRK DGVLVSTQDS RIKQLENGVL QIRYAKLGDT GRYTCIASTP SGEATWSAYI EVQEFGVPVQ PPRPTDPNLI PSAPSKPEVT DVSRNTVTLS WQPNLNSGAT PTSYIIEAFS HASGSSWQTV AENVKTETSA IKGLKPNAIY LFLVRAANAY GISDPSQISD PVKTQDVLPT SQGVDHKQVQ RELGNAVLHL HNPTVLSSSS IEVHWTVDQQ SQYIQGYKIL YRPSGANHGE SDWLVFEVRT PAKNSVVIPD LRKGVNYEIK ARPFFNEFQG ADSEIKFAKT LEEAPSAPPQ GVTVSKNDGN GTAILVSWQP PPEDTQNGMV QEYKVWCLGN ETRYHINKTV DGSTFSVVIP FLVPGIRYSV EVAASTGAGS GVKSEPQFIQ LDAHGNPVSP EDQVSLAQQI SDVVKQPAFI AGIGAACWII LMVFSIWLYR HRKKRNGLTS TYAGIRKVPS FTFTPTVTYQ RGGEAVSSGG RPGLLNISEP AAQPWLADTW PNTGNNHNDC SISCCTAGNG NSDSNLTTYS RPADCIANYN NQLDNKQTNL MLPESTVYGD VDLSNKINEM KTFNSPNLKD GRFVNPSGQP TPYATTQLIQ SNLSNNMNNG SGDSGEKHWK PLGQQKQEVA PVQYNIVEQN KLNKDYRAND TVPPTIPYNQ SYDQNTGGSY NSSDRGSSTS GSQGHKKGAR TPKVPKQGGM NWADLLPPPP AHPPPHSNSE EYNISVDESY DQEMPCPVPP ARMYLQQDEL EEEEDERGPT PPVRGAASSP AAVSYSHQST ATLTPSPQEE LQPMLQDCPE ETGHMQHQPD RRRQPVSPPP PPRPISPPHT YGYISGPLVS DMDTDAPEEE EDEADMEVAK MQTRRLLLRG LEQTPASSVG DLESSVTGSM INGWGSASEE DNISSGRSSV SSSDGSFFTD ADFAQAVAAA AEYAGLKVAR RQMQDAAGRR HFHASQCPRP TSPVSTDSNM SAAVMQKTRP AKKLKHQPGH LRRETYTDDL PPPPVPPPAI KSPTAQSKTQ LEVRPVVVPK LPSMDARTDR SSDRKGSSYK GREVLDGRQV VDMRTNPGDP REAQEQQNDG KGRGNKAAKR DLPPAKTHLI QEDILPYCRP TFPTSNNPRD PSSSSSMSSR GSGSRQREQA NVGRRNIAEM QVLGGYERGE DNNEELEETE S