PRK4_ARATH » Pollen receptor-like kinase 4

PRK4_ARATH » Pollen receptor-like kinase 4
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
PRK4_ARATH » Pollen receptor-like kinase 4 »
Hydrophobic Thickness 38.4 ± 4.6 Å
Tilt Angle 31 ± 18°
ΔGtransfer -36.1 kcal/mol
ΔGfold -31.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 275-303 (271-308)
Pathways none
PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 49-92, PDBID: 3RGX, Subunit A, Seq Identity:32%, Leucine rich repeat N-terminal domain

AA: 142-203, PDBID: 4BSR, Subunit B, Seq Identity:40%, Leucine rich repeat

AA: 373-643, PDBID: 3OMV, Subunit A, Seq Identity:32%, Protein tyrosine kinase

UniProt annotation for PRK4_ARATH » Pollen receptor-like kinase 4
FUNCTION: Receptor-like kinase involved in the control of pollen germination and pollen tube polar growth (PubMed). Can phosphorylate ROPGEF1 in vitro (PubMed).

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

SUBUNIT: Interacts in vitro with ROPGEF1 (via PRONE domain) (PubMed). Interacts weakly with the GRI peptide (PubMed).

TISSUE SPECIFICITY: Expressed in pollen and/or in flowers, but not in leaves.

DOMAIN: The protein kinase domain may be catalytically impaired due to the lack of the conserved Asp active site at position 500, which is replaced by a His residue.

UniProt features for PRK4_ARATH » Pollen receptor-like kinase 4
SIGNAL 1 39
CHAIN 40 679 Pollen receptor-like kinase 4.
REPEAT 118 141 LRR 1.
REPEAT 142 165 LRR 2.
REPEAT 167 191 LRR 3.
REPEAT 193 217 LRR 4.
REPEAT 234 257 LRR 5.
DOMAIN 372 646 Protein kinase.
Amino Acid Sequence for PRK4_ARATH » Pollen receptor-like kinase 4
MLTWETPVML ASNTASTKKL AFITTFLIIV LCPVTMVMSQ PQADVLPLPA SDADCLLRFK DTLVNASFIS SWDPSISPCK RNSENWFGVL CVTGNVWGLQ LEGMGLTGKL DLEPLAAIKN LRTLSFMNNK FNGSMPSVKN FGALKSLYLS NNRFTGEIPA DAFDGMHHLK KLLLANNAFR GSIPSSLAYL PMLLELRLNG NQFHGEIPYF KQKDLKLASF ENNDLEGPIP ESLSNMDPVS FSGNKNLCGP PLSPCSSDSG SSPDLPSSPT EKNKNQSFFI IAIVLIVIGI ILMIISLVVC ILHTRRRKSL SAYPSAGQDR TEKYNYDQST DKDKAADSVT SYTSRRGAVP DQNKLLFLQD DIQRFDLQDL LRASAEVLGS GSFGSSYKTG INSGQMLVVK RYKHMNNVGR DEFHEHMRRL GRLKHPNLLP IVAYYYRREE KLLIAEFMPN RSLASHLHAN HSVDQPGLDW PTRLKIIQGV AKGLGYLFNE LTTLTIPHGH LKSSNVVLDE SFEPLLTDYA LRPVMNSEQS HNLMISYKSP EYSLKGHLTK KTDVWCLGVL ILELLTGRFP ENYLSQGYDA NMSLVTWVSN MVKEKKTGDV FDKEMTGKKN CKAEMLNLLK IGLSCCEEDE ERRMEMRDAV EKIERLKEGE FDNDFASTTH NVFASRLIDD DDFGFAMNR