PRK3_ARATH » Pollen receptor-like kinase 3

PRK3_ARATH » Pollen receptor-like kinase 3
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
PRK3_ARATH » Pollen receptor-like kinase 3 »
Hydrophobic Thickness 35.2 ± 2.4 Å
Tilt Angle 18 ± 3°
ΔGtransfer -35.2 kcal/mol
ΔGfold -30.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 248-272 (247-276)
Pathways none
PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 24-63, PDBID: 3RGX, Subunit A, Seq Identity:33%, Leucine rich repeat N-terminal domain

AA: 114-175, PDBID: 4LI1, Subunit A, Seq Identity:34%, Leucine rich repeat

AA: 188-213, PDBID: 4M7E, Subunit C, Seq Identity:40%, Leucine Rich Repeat

AA: 359-628, PDBID: 4PL3, Subunit B, Seq Identity:37%, Protein kinase domain

UniProt annotation for PRK3_ARATH » Pollen receptor-like kinase 3
FUNCTION: Receptor-like kinase involved in the control of pollen germination and pollen tube polar growth (PubMed). Can phosphorylate ROPGEF1 in vitro (PubMed).

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

ENZYME REGULATION: The phosphorylation activity is calcium- independent.

SUBUNIT: Interacts in vitro with ROPGEF1 (via PRONE domain).

TISSUE SPECIFICITY: Expressed in pollen and/or in flowers, but not in leaves.

DOMAIN: The protein kinase domain may be catalytically impaired due to the lack of the conserved Asp active site at position 486, which is replaced by a Asn residue.

UniProt features for PRK3_ARATH » Pollen receptor-like kinase 3
SIGNAL 1 19
CHAIN 20 633 Pollen receptor-like kinase 3.
REPEAT 90 115 LRR 1.
REPEAT 117 137 LRR 2.
REPEAT 138 162 LRR 3.
REPEAT 163 186 LRR 4.
REPEAT 188 210 LRR 5.
DOMAIN 358 633 Protein kinase.
Amino Acid Sequence for PRK3_ARATH » Pollen receptor-like kinase 3
MTAVLFLCFL LICFSFTPSL QNVSESEPLV RFKRSVNITK GDLNSWRTGT DPCNGKWFGI YCQKGQTVSG IHVTRLGLSG TINIEDLKDL PNLRTIRLDN NLLSGPLPPF FKLPGLKSLL LSNNSFSGEI ADDFFKETPQ LKRVFLDNNR LSGKIPASLM QLAGLEELHM QGNQFTGEIP PLTDGNKVLK SLDLSNNDLE GEIPITISDR KNLEMKFEGN QRLCGSPLNI ECDEKPSSTG SGNEKNNTAK AIFMVILFLL IFLFVVAIIT RWKKKRQPEF RMLGKDHLSD QESVEVRVPD SIKKPIDSSK KRSNAEGSSK KGSSHNGKGA GGGPGSGMGD IIMVNSEKGS FGLPDLMKAA AEVLGNGSLG SAYKAVMANG LSVVVKRIRD MNKLAREAFD TEMQRFGKLR HPNVLTPLAY HYRREEKLVV SEYMPKSSLL YVLHGDRGVY HSELTWATRL KIIQGVARGM DFLHEEFASY DLPHGNLKSS NVLLSETYEP LISDYAFLPL LQPNNASQAL FAFKSPEFVQ NQQVSPKSDV YCLGIIVLEV MTGKFPSQYL NTGKGGTDIV EWVQSSIAQH KEEELIDPEI ASNTDSIKQM VELLRIGAAC IASNPNERQN MKEIVRRIER VTL