PMEL_HUMAN » Melanocyte protein PMEL

PMEL_HUMAN » Melanocyte protein PMEL
Magnify PMEL_HUMAN » Melanocyte protein PMELEnlarged view of image
3D view in GLMol or JMol

gray dot

Download Coordinates

gray dot

Topology in Melanosome membrane
Topologymelanosome lumenal side
cytoplasmic side
PMEL_HUMAN » Melanocyte protein PMEL » ME20-M;ME20M; Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;
Hydrophobic Thickness 33.4 ± 1.7 Å
Tilt Angle 12 ± 0°
ΔGtransfer -26.4 kcal/mol
ΔGfold -23.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Out
TM Segments 595-616 (595-621)
Pathways none
PDB 1tvb (C/F=209-217), 1tvh (C/F=209-217), 3cc5 (C/F=25-33), 4is6 (C=44-59)
OPM none
Complexes

PMEL:2B14:DRA_HUMAN

PMEL:1A02:B2MG_HUMAN

Interactions

1A02, Complex: PMEL:1A02:B2MG, PDBID: 1TVB

2B14, Complex: PMEL:2B14:DRA, PDBID: 4IS6

Domains

AA: 225-301, PDBID: 2C26, Subunit A, Seq Identity:25%, PKD domain

UniProt annotation for PMEL_HUMAN » Melanocyte protein PMEL
FUNCTION: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

SUBUNIT: Heterooligomer; disulfide-linked heterooligomers of M- alpha and M-beta. Interacts with MLANA. Interacts (via luminal domain) with CD63; this is important for normal sorting of the luminal domain after proteolytic processing.

TISSUE SPECIFICITY: Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

DOMAIN: The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.

DOMAIN: The lumenal domain is necessary for correct processing and trafficking to melanosomes.

UniProt features for PMEL_HUMAN » Melanocyte protein PMEL
SIGNAL 1 24
CHAIN 25 661 Melanocyte protein PMEL.
CHAIN 25 467 M-alpha.
CHAIN 470 661 M-beta.
DOMAIN 255 292 PKD.
REPEAT 315 327 1.
REPEAT 328 340 2.
REPEAT 341 353 3.
REPEAT 354 366 4.
REPEAT 367 379 5.
REPEAT 380 392 6.
REPEAT 393 405 7.
REPEAT 406 418 8.
REPEAT 419 431 9.
REPEAT 432 444 10.
REGION 315 444 10 X 13 AA approximate tandem repeats, RPT domain.
Amino Acid Sequence for PMEL_HUMAN » Melanocyte protein PMEL
MDLVLKRCLL HLAVIGALLA VGATKVPRNQ DWLGVSRQLR TKAWNRQLYP EWTEAQRLDC WRGGQVSLKV SNDGPTLIGA NASFSIALNF PGSQKVLPDG QVIWVNNTII NGSQVWGGQP VYPQETDDAC IFPDGGPCPS GSWSQKRSFV YVWKTWGQYW QVLGGPVSGL SIGTGRAMLG THTMEVTVYH RRGSRSYVPL AHSSSAFTIT DQVPFSVSVS QLRALDGGNK HFLRNQPLTF ALQLHDPSGY LAEADLSYTW DFGDSSGTLI SRALVVTHTY LEPGPVTAQV VLQAAIPLTS CGSSPVPGTT DGHRPTAEAP NTTAGQVPTT EVVGTTPGQA PTAEPSGTTS VQVPTTEVIS TAPVQMPTAE STGMTPEKVP VSEVMGTTLA EMSTPEATGM TPAEVSIVVL SGTTAAQVTT TEWVETTARE LPIPEPEGPD ASSIMSTESI TGSLGPLLDG TATLRLVKRQ VPLDCVLYRY GSFSVTLDIV QGIESAEILQ AVPSGEGDAF ELTVSCQGGL PKEACMEISS PGCQPPAQRL CQPVLPSPAC QLVLHQILKG GSGTYCLNVS LADTNSLAVV STQLIMPGQE AGLGQVPLIV GILLVLMAVV LASLIYRRRL MKQDFSVPQL PHSSSHWLRL PRIFCSCPIG ENSPLLSGQQ V