PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1

PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 » Csk-binding protein;Transmembrane adapter protein PAG;Transmembrane phosphoprotein Cbp;
Hydrophobic Thickness 34.2 ± 1.7 Å
Tilt Angle 1 ± 12°
ΔGtransfer -29.4 kcal/mol
ΔGfold -25.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Out
TM Segments 16-38 (10-44)
Pathways

Immune System (Reactome)

Proteasome (KEGG)

Signal Transduction (Reactome)

PDB none
OPM none
Complexes none
Interactions

EGFR, Complex: PHAG1:EGFR

HTRA2, Complex: HTRA2:PHAG1, PubMed

INAR2, Complex: PHAG1:INAR2, PubMed

LYRIC, Complex: LYRIC:PHAG1, PubMed

Domains

AA: 2-432, PDBID: 2RSY, Subunit B, Seq Identity:77%, Phosphoprotein associated with glycosphingolipid-enriched

UniProt annotation for PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
FUNCTION: Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

SUBUNIT: Interacts with FYN. When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with LYN on plasma membrane lipid rafts. Identified in a complex with LYN and STAT3.

TISSUE SPECIFICITY: Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).

UniProt features for PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
CHAIN 1 432 Phosphoprotein associated with glycosphingolipid-enriched microdomains 1.
REGION 317 320 Interaction with CSK.
REGION 430 432 Interaction with SLC9A3R1.
Amino Acid Sequence for PHAG1_HUMAN » Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
MGPAGSLLGS GQMQITLWGS LAAVAIFFVI TFLIFLCSSC DREKKPRQHS GDHENLMNVP SDKEMFSRSV TSLATDAPAS SEQNGALTNG DILSEDSTLT CMQHYEEVQT SASDLLDSQD STGKPKCHQS RELPRIPPES AVDTMLTARS VDGDQGLGME GPYEVLKDSS SQENMVEDCL YETVKEIKEV AAAAHLEKGH SGKAKSTSAS KELPGPQTEG KAEFAEYASV DRNKKCRQSV NVESILGNSC DPEEEAPPPV PVKLLDENEN LQEKEGGEAE ESATDTTSET NKRFSSLSYK SREEDPTLTE EEISAMYSSV NKPGQLVNKS GQSLTVPEST YTSIQGDPQR SPSSCNDLYA TVKDFEKTPN STLPPAGRPS EEPEPDYEAI QTLNREEEKA TLGTNGHHGL VPKENDYESI SDLQQGRDIT RL