NYV1_YEAST » Vacuolar v-SNARE NYV1

NYV1_YEAST » Vacuolar v-SNARE NYV1
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Topology in Vacuolar membrane
Topologyvacuole lumenal side
cytoplasmic side
NYV1_YEAST » Vacuolar v-SNARE NYV1 » R-SNARE NYV1; New v-SNARE 1;Synaptobrevin NYV1;
Hydrophobic Thickness 31.2 ± 4.2 Å
Tilt Angle 5 ± 13°
ΔGtransfer -26.9 kcal/mol
ΔGfold -18.4 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 232-252 (226-253)
Pathways none
PDB 2fz0 (1-149)
OPM none
Complexes none
Interactions

BET1, Complex: BET1:NYV1, PubMed

SEC22, Complex: SEC22:NYV1, PubMed

SED5, Complex: SED5:NYV1, PubMed

SSO1, Complex: SSO1:NYV1

STX8, Complex: STX8:NYV1, PubMed

TLG1, Complex: TLG1:NYV1, PubMed

VAM3, Complex: VAM3:NYV1, PubMed

VTI1, Complex: VTI1:NYV1, PubMed

Domains

AA: 6-144, PDBID: 2FZ0, Subunit A, Seq Identity:100%, Vacuolar R-SNARE Nyv1 N terminal

AA: 165-252, PDBID: 5BV0, Subunit C, Seq Identity:39%, Synaptobrevin

UniProt annotation for NYV1_YEAST » Vacuolar v-SNARE NYV1
FUNCTION: Vacuolar v-SNARE required for docking. Only involved in homotypic vacuole fusion. Required for Ca(2+) efflux from the vacuolar lumen, a required signal for subsequent membrane fusion events, by inhibiting vacuolar Ca(2+)-ATPase PMC1 and promoting Ca(2+) release when forming trans-SNARE assemblies during the docking step.

SUBUNIT: Present in a pentameric cis-SNARE complex composed of the v-SNAREs NYV1, VTI1 and YKT6, and the t-SNAREs VAM3 and VAM7 on vacuolar membranes. Interacts in trans with the cognate t-SNARE VAM3 during the docking step of homotypic vacuolar fusion. Interacts with the vacuolar transporter chaperone (VTC) complex and the vacuolar Ca(2+)-ATPase PMC1.

MISCELLANEOUS: Present with 1885 molecules/cell in log phase SD medium.

UniProt features for NYV1_YEAST » Vacuolar v-SNARE NYV1
CHAIN 1 253 Vacuolar v-SNARE NYV1.
DOMAIN 167 227 v-SNARE coiled-coil homology.
Amino Acid Sequence for NYV1_YEAST » Vacuolar v-SNARE NYV1
MKRFNVSYVE VIKNGETISS CFQPFQKNEN YGTITSANEQ ITPVIFHNLI MDMVLPKVVP IKGNKVTKMS MNLIDGFDCF YSTDDHDPKT VYVCFTLVDM PKILPIRILS GLQEYESNAT NELLSSHVGQ ILDSFHEELV EYRNQTLNSS GNGQSSNGNG QNTISDIGDA TEDQIKDVIQ IMNDNIDKFL ERQERVSLLV DKTSQLNSSS NKFRRKAVNI KEIMWWQKVK NITLLTFTII LFVSAAFMFF YLW