NKG2D_HUMAN » NKG2-D type II integral membrane protein

NKG2D_HUMAN » NKG2-D type II integral membrane protein
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
NKG2D_HUMAN » NKG2-D type II integral membrane protein » Killer cell lectin-like receptor subfamily K member 1;NK cell receptor D;NKG2-D-activating NK receptor;
Hydrophobic Thickness 36.0 ± 0.8 Å
Tilt Angle 0 ± 0°
ΔGtransfer -38.4 kcal/mol
ΔGfold -14.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology In
TM Segments 52-76 (52-80)
Pathways

Immune System (Reactome)

Malaria (KEGG)

Natural killer cell mediated cytotoxicity (KEGG)

PDB 4pdc (A/B/C/D=93-215), 1hyr (A/B=80-216), 1kcg (A/B=93-216), 1mpu (A=80-216)
OPM none
Complexes

MICA:NKG2D_HUMAN

Interactions

HCST, Complex: HCST:NKG2D, PubMed

MICA, Complex: MICA:NKG2D, PDBID: 1hyr

MICB, Complex: NKG2D:MICB, PubMed

N2DL4, Complex: N2DL4:NKG2D, PubMed

RET1G, Complex: NKG2D:RET1G, PubMed

TYOBP, Complex: NKG2D:TYOBP, PubMed

Domains

AA: 116-213, PDBID: 1HYR, Subunit A, Seq Identity:100%, Lectin C-type domain

UniProt annotation for NKG2D_HUMAN » NKG2-D type II integral membrane protein
FUNCTION: Function as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

SUBUNIT: Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Does not interact with TYROBP.

TISSUE SPECIFICITY: Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).

INDUCTION: Up-regulated by interleukin IL15 in primary NK cells.

MISCELLANEOUS: Is not capable of signal transduction by itself, but operates through the adapter protein HCST (PubMed and PubMed). Some families of ligands for human and mouse KLRK1 receptors have been characterized being very similar in structure and highly likely to be orthologs. In humans, an additional distinct subfamily of ligands (MICA and MICB) differs structurally, having an extra MHC alpha 3-like domain (PubMed).

UniProt features for NKG2D_HUMAN » NKG2-D type II integral membrane protein
CHAIN 1 216 NKG2-D type II integral membrane protein.
DOMAIN 98 213 C-type lectin.
DISULFID 96 105
DISULFID 99 110
DISULFID 127 211
DISULFID 189 203
Amino Acid Sequence for NKG2D_HUMAN » NKG2-D type II integral membrane protein
MGWIRGRRSR HSWEMSEFHN YNLDLKKSDF STRWQKQRCP VVKSKCRENA SPFFFCCFIA VAMGIRFIIM VAIWSAVFLN SLFNQEVQIP LTESYCGPCP KNWICYKNNC YQFFDESKNW YESQASCMSQ NASLLKVYSK EDQDLLKLVK SYHWMGLVHI PTNGSWQWED GSILSPNLLT IIEMQKGDCA LYASSFKGYI ENCSTPNTYI CMQRTV