NIK3_ARATH » Protein NSP-INTERACTING KINASE 3

NIK3_ARATH » Protein NSP-INTERACTING KINASE 3
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
NIK3_ARATH » Protein NSP-INTERACTING KINASE 3 » LRR receptor-like serine/threonine-protein kinase NIK3;
Hydrophobic Thickness 41.2 ± 1.8 Å
Tilt Angle 1 ± 1°
ΔGtransfer -47.2 kcal/mol
ΔGfold -21.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 241-263 (235-267)
Pathways none
PDB none
OPM none
Complexes none
Interactions

PAM74, Complex: NIK3:PAM74

Domains

AA: 32-72, PDBID: 4M7E, Subunit D, Seq Identity:52%, Leucine rich repeat N-terminal domain

AA: 123-145, PDBID: 4MN8, Subunit A, Seq Identity:54%, Leucine Rich Repeat

AA: 301-574, PDBID: 3TL8, Subunit A, Seq Identity:74%, Protein kinase domain

UniProt annotation for NIK3_ARATH » Protein NSP-INTERACTING KINASE 3
FUNCTION: Involved in defense response to geminivirus infection.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

ENZYME REGULATION: Inhibited by the viral nuclear shuttle protein (NSP) that binds to the region required for oligomerization.

SUBUNIT: Oligomer. Interacts with geminivirus nuclear shuttle protein (NSP).

TISSUE SPECIFICITY: Expressed in seedlings, leaves and flowers.

UniProt features for NIK3_ARATH » Protein NSP-INTERACTING KINASE 3
SIGNAL 1 25 Potential.
CHAIN 26 632 Protein NSP-INTERACTING KINASE 3.
REPEAT 97 121 LRR 1.
REPEAT 122 145 LRR 2.
REPEAT 147 168 LRR 3.
REPEAT 169 193 LRR 4.
DOMAIN 301 584 Protein kinase.
REGION 415 495 Interaction with geminivirus NSP protein (By similarity).
ACT_SITE 428 428 Proton acceptor (By similarity).
Amino Acid Sequence for NIK3_ARATH » Protein NSP-INTERACTING KINASE 3
MEGVRFVVWR LGFLVFVWFF DISSATLSPT GVNYEVTALV AVKNELNDPY KVLENWDVNS VDPCSWRMVS CTDGYVSSLD LPSQSLSGTL SPRIGNLTYL QSVVLQNNAI TGPIPETIGR LEKLQSLDLS NNSFTGEIPA SLGELKNLNY LRLNNNSLIG TCPESLSKIE GLTLVDISYN NLSGSLPKVS ARTFKVIGNA LICGPKAVSN CSAVPEPLTL PQDGPDESGT RTNGHHVALA FAASFSAAFF VFFTSGMFLW WRYRRNKQIF FDVNEQYDPE VSLGHLKRYT FKELRSATNH FNSKNILGRG GYGIVYKGHL NDGTLVAVKR LKDCNIAGGE VQFQTEVETI SLALHRNLLR LRGFCSSNQE RILVYPYMPN GSVASRLKDN IRGEPALDWS RRKKIAVGTA RGLVYLHEQC DPKIIHRDVK AANILLDEDF EAVVGDFGLA KLLDHRDSHV TTAVRGTVGH IAPEYLSTGQ SSEKTDVFGF GILLLELITG QKALDFGRSA HQKGVMLDWV KKLHQEGKLK QLIDKDLNDK FDRVELEEIV QVALLCTQFN PSHRPKMSEV MKMLEGDGLA ERWEATQNGT GEHQPPPLPP GMVSSSPRVR YYSDYIQESS LVVEAIELSG PR