NIK2_ARATH » Protein NSP-INTERACTING KINASE 2

NIK2_ARATH » Protein NSP-INTERACTING KINASE 2
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
NIK2_ARATH » Protein NSP-INTERACTING KINASE 2 » LRR receptor-like serine/threonine-protein kinase NIK2;
Hydrophobic Thickness 41.6 ± 5.0 Å
Tilt Angle 1 ± 2°
ΔGtransfer -25.4 kcal/mol
ΔGfold -19.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 245-272 (242-275)
Pathways none
PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 39-79, PDBID: 4M7E, Subunit D, Seq Identity:46%, Leucine rich repeat N-terminal domain

AA: 129-189, PDBID: 4MN8, Subunit A, Seq Identity:44%, Leucine rich repeat

AA: 312-581, PDBID: 3TL8, Subunit A, Seq Identity:67%, Protein kinase domain

UniProt annotation for NIK2_ARATH » Protein NSP-INTERACTING KINASE 2
FUNCTION: Involved in defense response to geminivirus infection (By similarity). Phosphorylates RPL10A in vitro.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

ENZYME REGULATION: Inhibited by the viral nuclear shuttle protein (NSP) that binds to the region required for oligomerization.

SUBUNIT: Oligomer. Interacts with geminivirus nuclear shuttle protein (NSP).

TISSUE SPECIFICITY: Expressed in flowers and roots.

UniProt features for NIK2_ARATH » Protein NSP-INTERACTING KINASE 2
SIGNAL 1 32 Potential.
CHAIN 33 635 Protein NSP-INTERACTING KINASE 2.
REPEAT 104 128 LRR 1.
REPEAT 129 153 LRR 2.
REPEAT 155 176 LRR 3.
REPEAT 177 200 LRR 4.
DOMAIN 312 591 Protein kinase.
REGION 422 502 Interaction with geminivirus NSP protein (By similarity).
ACT_SITE 435 435 Proton acceptor (By similarity).
Amino Acid Sequence for NIK2_ARATH » Protein NSP-INTERACTING KINASE 2
MLQGRREAKK SYALFSSTFF FFFICFLSSS SAELTDKGVN FEVVALIGIK SSLTDPHGVL MNWDDTAVDP CSWNMITCSD GFVIRLEAPS QNLSGTLSSS IGNLTNLQTV LLQNNYITGN IPHEIGKLMK LKTLDLSTNN FTGQIPFTLS YSKNLQYLRV NNNSLTGTIP SSLANMTQLT FLDLSYNNLS GPVPRSLAKT FNVMGNSQIC PTGTEKDCNG TQPKPMSITL NSSQNKSSDG GTKNRKIAVV FGVSLTCVCL LIIGFGFLLW WRRRHNKQVL FFDINEQNKE EMCLGNLRRF NFKELQSATS NFSSKNLVGK GGFGNVYKGC LHDGSIIAVK RLKDINNGGG EVQFQTELEM ISLAVHRNLL RLYGFCTTSS ERLLVYPYMS NGSVASRLKA KPVLDWGTRK RIALGAGRGL LYLHEQCDPK IIHRDVKAAN ILLDDYFEAV VGDFGLAKLL DHEESHVTTA VRGTVGHIAP EYLSTGQSSE KTDVFGFGIL LLELITGLRA LEFGKAANQR GAILDWVKKL QQEKKLEQIV DKDLKSNYDR IEVEEMVQVA LLCTQYLPIH RPKMSEVVRM LEGDGLVEKW EASSQRAETN RSYSKPNEFS SSERYSDLTD DSSVLVQAME LSGPR