I12R2_HUMAN » Interleukin-12 receptor subunit beta-2

I12R2_HUMAN » Interleukin-12 receptor subunit beta-2
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
I12R2_HUMAN » Interleukin-12 receptor subunit beta-2 » IL-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12R-beta-2; IL-12RB2;
Hydrophobic Thickness 32.8 ± 3.0 Å
Tilt Angle 10 ± 4°
ΔGtransfer -36.2 kcal/mol
ΔGfold -14.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Out
TM Segments 623-644 (623-649)
Pathways

Cytokine-cytokine receptor interaction (KEGG)

Jak-STAT signaling pathway (KEGG)

PDB none
OPM none
Complexes none
Interactions

I12R1, Complex: I12R2:I12R1, PubMed

Domains

AA: 28-110, PDBID: 2D9Q, Subunit B, Seq Identity:19%, Ig-like C2-type domain

AA: 422-511, PDBID: 2ED8, Subunit A, Seq Identity:26%, Fibronectin type III domain

UniProt annotation for I12R2_HUMAN » Interleukin-12 receptor subunit beta-2
FUNCTION: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production.

SUBUNIT: Heterodimer/heterooligomer; disulfide-linked. The functional high affinity IL12 receptor is composed of I12RB1 and IL12RB2. Il12RB2 binds JAK2 (via its N-terminal) through a membrane-proximal region of the cytoplasmic domain. Interaction, in vitro and in vivo, with SOCS3 (via its SH2 domain) inhibits the STAT4-mediated activation. Binds STAT4 through a membrane-distal C-terminal region.

TISSUE SPECIFICITY: Isoform 2 is expressed at similar levels in both naive and activated T-cells.

DEVELOPMENTAL STAGE: Maximum levels in Th1 cells between day 3 and day 8 of activation.

INDUCTION: In vitro, up-regulated by IFN-alpha.

DOMAIN: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell- surface receptor binding.

DOMAIN: The box 1 motif is required for JAK interaction and/or activation.

UniProt features for I12R2_HUMAN » Interleukin-12 receptor subunit beta-2
SIGNAL 1 23 Potential.
CHAIN 24 862 Interleukin-12 receptor subunit beta-2.
DOMAIN 124 218 Fibronectin type-III 1.
DOMAIN 224 316 Fibronectin type-III 2.
DOMAIN 317 415 Fibronectin type-III 3.
DOMAIN 420 517 Fibronectin type-III 4.
DOMAIN 521 617 Fibronectin type-III 5.
REGION 796 801 Required for STAT4 binding.
MOTIF 305 309 WSXWS motif.
MOTIF 662 670 Box 1 motif.
Amino Acid Sequence for I12R2_HUMAN » Interleukin-12 receptor subunit beta-2
MAHTFRGCSL AFMFIITWLL IKAKIDACKR GDVTVKPSHV ILLGSTVNIT CSLKPRQGCF HYSRRNKLIL YKFDRRINFH HGHSLNSQVT GLPLGTTLFV CKLACINSDE IQICGAEIFV GVAPEQPQNL SCIQKGEQGT VACTWERGRD THLYTEYTLQ LSGPKNLTWQ KQCKDIYCDY LDFGINLTPE SPESNFTAKV TAVNSLGSSS SLPSTFTFLD IVRPLPPWDI RIKFQKASVS RCTLYWRDEG LVLLNRLRYR PSNSRLWNMV NVTKAKGRHD LLDLKPFTEY EFQISSKLHL YKGSWSDWSE SLRAQTPEEE PTGMLDVWYM KRHIDYSRQQ ISLFWKNLSV SEARGKILHY QVTLQELTGG KAMTQNITGH TSWTTVIPRT GNWAVAVSAA NSKGSSLPTR INIMNLCEAG LLAPRQVSAN SEGMDNILVT WQPPRKDPSA VQEYVVEWRE LHPGGDTQVP LNWLRSRPYN VSALISENIK SYICYEIRVY ALSGDQGGCS SILGNSKHKA PLSGPHINAI TEEKGSILIS WNSIPVQEQM GCLLHYRIYW KERDSNSQPQ LCEIPYRVSQ NSHPINSLQP RVTYVLWMTA LTAAGESSHG NEREFCLQGK ANWMAFVAPS ICIAIIMVGI FSTHYFQQKV FVLLAALRPQ WCSREIPDPA NSTCAKKYPI AEEKTQLPLD RLLIDWPTPE DPEPLVISEV LHQVTPVFRH PPCSNWPQRE KGIQGHQASE KDMMHSASSP PPPRALQAES RQLVDLYKVL ESRGSDPKPE NPACPWTVLP AGDLPTHDGY LPSNIDDLPS HEAPLADSLE ELEPQHISLS VFPSSSLHPL TFSCGDKLTL DQLKMRCDSL ML