HAP2_ARATH » Protein HAPLESS 2

HAP2_ARATH » Protein HAPLESS 2
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
HAP2_ARATH » Protein HAPLESS 2 » GENERATIVE CELL SPECIFIC 1;
Hydrophobic Thickness 34.4 ± 2.0 Å
Tilt Angle 0 ± 1°
ΔGtransfer -40.7 kcal/mol
ΔGfold -22.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 553-583 (553-585)
Pathways none
PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 281-328, Male gamete fusion factor

UniProt annotation for HAP2_ARATH » Protein HAPLESS 2
FUNCTION: Involved in male fertility. Essential for pollen tube guidance, successful gamete attachment and fertilization.

TISSUE SPECIFICITY: Expressed only in mature pollen, in the two sperm cells.

DEVELOPMENTAL STAGE: Expressed during pollen development and tube growth.

DOMAIN: The N-terminal domain (62-541)and the C-terminal domain (583-705) are both required for functional gamete fusion. A positive charge, and not a conserved primary amino acid sequence, is required for a functional C-terminal domain. The N-terminal domain may be involved in interactions with another membrane-bound protein on female gametes, while positively charged C-terminus may function through electrostatic interactions with the sperm plasma membrane.

UniProt features for HAP2_ARATH » Protein HAPLESS 2
SIGNAL 1 24 Potential.
CHAIN 25 705 Protein HAPLESS 2.
Amino Acid Sequence for HAP2_ARATH » Protein HAPLESS 2
MVNAILMACI LAGIFVGMFN EVDGIQILSK SKLEKCEKTS DSGNLNCSTK IVLNLAVPSG SSGGEASIVA EIVEVEDNSS SNMQTVRIPP VITVNKSAAY ALYDLTYIRD VPYKPQEYHV TTRKCEHDAG PDIVQICERL RDEKGNVLEQ TQPICCPCGP QRRMPSSCGD IFDKMIKGKA NTAHCLRFPG DWFHVFGIGQ RSLGFSVRVE LKTGTRVSEV IIGPENRTAT ANDNFLKVNL IGDFGGYTSI PSFEDFYLVI PREAAEAGQP GSLGANYSMW MLLERVRFTL DGLECNKIGV GYEAFNTQPN FCSSPYWSCL HNQLWNFRES DINRIDRHQL PLYGLEGRFE RINQHPNAGP HSFSIGVTET LNTNLMIELR ADDIEYVFQR SPGKIINIAI PTFEALTQFG VAAVIIKNTG EVEASYSLTF DCSKGVAFVE EQFFIIKPKA VTTRSFKLYP TKDQAAKYIC TAILKDSQFS EVDRAECQFS TTATVLDNGT QVTNPFQIPE TQPKGFFDSI RILWTKIING LVDFITGDTC RNKCSSFFDF SCHIQYVCLS WMVMFGLLLA LFPITCLLLW LLHQKGLFDP CYDWWEDHFD LDHHRRLLPS RADVVNRHHH HHKHRHHHNH HRRTHQRHKH HHGQDDDVLQ KMMLERDHSD SHYYHQLHRV HKDSKQKQRR RAKHGIVLPR DVHVERQRKQ RLRES