GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4

GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4
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Topology in Golgi apparatus membrane
TopologyGolgi lumenal side
cytoplasmic side
GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4 » Core 2-branching enzyme 3;Core2-GlcNAc-transferase 3;C2GnT3;
Hydrophobic Thickness 28.4 ± 3.7 Å
Tilt Angle 18 ± 0°
ΔGtransfer -20.5 kcal/mol
ΔGfold -12.3 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology In
TM Segments 12-32 (11-35)
Pathways

Mucin type O-Glycan biosynthesis (KEGG)

PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 133-402, PDBID: 2GAK, Subunit B, Seq Identity:44%, Core-2/I-Branching enzyme

UniProt annotation for GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4
FUNCTION: Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity.

CATALYTIC ACTIVITY: UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D- galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D- galactosaminyl-R.

TISSUE SPECIFICITY: Predominantly expressed in thymus. Weakly expressed in pancreas, peripheral blood leukocytes, placenta, small intestine and stomach. Barely detectable in liver, spleen, lung and lymph node.

UniProt features for GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4
CHAIN 1 453 Beta-1,3-galactosyl-O-glycosyl- glycoprotein beta-1,6-N- acetylglucosaminyltransferase 4.
DISULFID 73 227 By similarity.
DISULFID 161 382 By similarity.
DISULFID 182 209 By similarity.
DISULFID 391 423 By similarity.
Amino Acid Sequence for GCNT4_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 4
MKIFKCYFKH TLQQKVFILF LTLWLLSLLK LLNVRRLFPQ KDIYLVEYSL STSPFVRNRY THVKDEVRYE VNCSGIYEQE PLEIGKSLEI RRRDIIDLED DDVVAMTSDC DIYQTLRGYA QKLVSKEEKS FPIAYSLVVH KDAIMVERLI HAIYNQHNIY CIHYDRKAPD TFKVAMNNLA KCFSNIFIAS KLEAVEYAHI SRLQADLNCL SDLLKSSIQW KYVINLCGQD FPLKSNFELV SELKKLNGAN MLETVKPPNS KLERFTYHHE LRRVPYEYVK LPIRTNISKE APPHNIQIFV GSAYFVLSQA FVKYIFNNSI VQDFFAWSKD TYSPDEHFWA TLIRVPGIPG EISRSAQDVS DLQSKTRLVK WNYYEGFFYP SCTGSHLRSV CIYGAAELRW LIKDGHWFAN KFDSKVDPIL IKCLAEKLEE QQRDWITLPS EKLFMDRNLT TTS