GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3

GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
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Topology in Golgi apparatus membrane
TopologyGolgi lumenal side
cytoplasmic side
GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3 » C2GnT-mucin type;C2GnT-M; hC2GnT-M; Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase;C2/4GnT;
Hydrophobic Thickness 26.4 ± 4.2 Å
Tilt Angle 1 ± 1°
ΔGtransfer -20.9 kcal/mol
ΔGfold -12.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology In
TM Segments 12-33 (8-34)
Pathways

Mucin type O-Glycan biosynthesis (KEGG)

PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 133-402, PDBID: 2GAK, Subunit B, Seq Identity:53%, Core-2/I-Branching enzyme

UniProt annotation for GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
FUNCTION: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development.

CATALYTIC ACTIVITY: UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D- galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D- galactosaminyl-R.

CATALYTIC ACTIVITY: UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D- glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

TISSUE SPECIFICITY: Primarily expressed in mucus-secreting tissues. Expressed in colon, kidney, small intestine, trachea, and stomach, where mucin is produced.

INDUCTION: By all-trans retinoic acid (ATRA), TNF and IL13/interleukin-13. Strongly down-regulated in colorectal cancer.

UniProt features for GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
CHAIN 1 438 Beta-1,3-galactosyl-O-glycosyl- glycoprotein beta-1,6-N- acetylglucosaminyltransferase 3.
DISULFID 70 227 By similarity.
DISULFID 161 382 By similarity.
DISULFID 182 209 By similarity.
DISULFID 391 423 By similarity.
Amino Acid Sequence for GCNT3_HUMAN » Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
MVQWKRLCQL HYLWALGCYM LLATVALKLS FRLKCDSDHL GLESRESQSQ YCRNILYNFL KLPAKRSINC SGVTRGDQEA VLQAILNNLE VKKKREPFTD THYLSLTRDC EHFKAERKFI QFPLSKEEVE FPIAYSMVIH EKIENFERLL RAVYAPQNIY CVHVDEKSPE TFKEAVKAII SCFPNVFIAS KLVRVVYASW SRVQADLNCM EDLLQSSVPW KYFLNTCGTD FPIKSNAEMV QALKMLNGRN SMESEVPPKH KETRWKYHFE VVRDTLHLTN KKKDPPPYNL TMFTGNAYIV ASRDFVQHVL KNPKSQQLIE WVKDTYSPDE HLWATLQRAR WMPGSVPNHP KYDISDMTSI ARLVKWQGHE GDIDKGAPYA PCSGIHQRAI CVYGAGDLNW MLQNHHLLAN KFDPKVDDNA LQCLEEYLRY KAIYGTEL