FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase

FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase
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Topology in Golgi apparatus membrane
TopologyGolgi lumenal side
cytoplasmic side
FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase » Alpha1-6FucT; Fucosyltransferase 8;GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase;GDP-fucose--glycoprotein fucosyltransferase;Glycoprotein 6-alpha-L-fucosyltransferase;
Hydrophobic Thickness 31.2 ± 2.0 Å
Tilt Angle 8 ± 6°
ΔGtransfer -22.3 kcal/mol
ΔGfold -12.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC, Reactome, HMDB
Topology In
TM Segments 8-29 (5-31)
Pathways

Glycosaminoglycan biosynthesis - keratan sulfate (KEGG)

N-Glycan biosynthesis (KEGG)

Transcriptional misregulation in cancer (KEGG)

Various types of N-glycan biosynthesis (KEGG)

PDB 2de0 (X=68-575)
OPM none
Complexes none
Interactions none
Domains

AA: 509-559, PDBID: 2DE0, Subunit X, Seq Identity:100%, Variant SH3 domain

UniProt annotation for FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase
FUNCTION: Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.

CATALYTIC ACTIVITY: GDP-beta-L-fucose + N(4)-(N-acetyl-beta-D- glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D- glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl- (1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D- glucosaminyl)asparagine = GDP + N(4)-(N-acetyl-beta-D- glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-(N-acetyl-beta-D- glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6))-beta-D-mannosyl- (1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-(alpha-L-fucosyl- (1->6))-N-acetyl-beta-D-glucosaminyl)asparagine.

UniProt features for FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase
CHAIN 1 575 Alpha-(1,6)-fucosyltransferase.
DOMAIN 206 493 GT23.
DOMAIN 502 563 SH3.
REGION 365 366 Important for donor substrate binding.
MOTIF 299 305 SH3-binding (Potential).
Amino Acid Sequence for FUT8_HUMAN » Alpha-(1,6)-fucosyltransferase
MRPWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL ERLKQQNEDL RRMAESLRIP EGPIDQGPAI GRVRVLEEQL VKAKEQIENY KKQTRNGLGK DHEILRRRIE NGAKELWFFL QSELKKLKNL EGNELQRHAD EFLLDLGHHE RSIMTDLYYL SQTDGAGDWR EKEAKDLTEL VQRRITYLQN PKDCSKAKKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT LILESQNWRY ATGGWETVFR PVSETCTDRS GISTGHWSGE VKDKNVQVVE LPIVDSLHPR PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD KKRVYLATDD PSLLKEAKTK YPNYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ TLHPDASANF HSLDDIYYFG GQNAHNQIAI YAHQPRTADE IPMEPGDIIG VAGNHWDGYS KGVNRKLGRT GLYPSYKVRE KIETVKYPTY PEAEK