EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1

EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1 » Extra sporogenous cells protein;Protein EXCESS MICROSPOROCYTES 1;
Hydrophobic Thickness 35.6 ± 2.8 Å
Tilt Angle 19 ± 15°
ΔGtransfer -28.7 kcal/mol
ΔGfold -20.2 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 827-851 (827-859)
Pathways none
PDB none
OPM none
Complexes none
Interactions

BETL2, Complex: EXS:BETL2

PAM74, Complex: EXS:PAM74

WAK3, Complex: EXS:WAK3

Domains

AA: 23-63, PDBID: 3rgx, Subunit A, Seq Identity:37%, Leucine rich repeat N-terminal domain

AA: 89-149, PDBID: 2z64, Subunit A, Seq Identity:32%, Leucine rich repeat

AA: 163-185, PDBID: 1ogq, Subunit A, Seq Identity:56%, Leucine Rich Repeat

AA: 235-257, PDBID: 1ogq, Subunit A, Seq Identity:44%, Leucine Rich Repeat

AA: 281-304, PDBID: 2z64, Subunit A, Seq Identity:34%, Leucine Rich repeat

AA: 473-495, PDBID: 1ogq, Subunit A, Seq Identity:40%, Leucine Rich Repeat

AA: 519-545, PDBID: 2z64, Subunit A, Seq Identity:30%, Leucine Rich repeat

AA: 605-664, PDBID: 2z64, Subunit A, Seq Identity:31%, Leucine rich repeat

AA: 917-1189, PDBID: 1z9i, Subunit A, Seq Identity:31%, Protein tyrosine kinase

UniProt annotation for EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1
FUNCTION: Receptor with a serine/threonine-protein kinase activity required for the specification of the correct number of male archesporial initials and for the subsequent specification of tapetal and middle cell layer identities. In seeds, required for enhancing cell size and the rate of embryonic development.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

SUBUNIT: Interacts with TPD1.

TISSUE SPECIFICITY: Present in young buds, open flowers and siliques but absent from mature leaves and roots. Strongly expressed in the young organ primordia, and as the anthers and ovules developed, became focused in the microsporangia and in the distal and chalazal regions of the ovule. In cv. Landsberg erecta, only expressed in the anthers of young floral buds.

DEVELOPMENTAL STAGE: Expressed during the differentiation of microsporocytes and tapetal cells. Also expressed in the meiocytes and young pollen grains until pollen mitosis II.

MISCELLANEOUS: Some ecotypic variation may occur: in cv. Landsberg erecta, meiocytes of a null mutant fail to undergo cytokinesis while in cv. C24, cytokinesis clearly takes place, with the mutant meiocytes degenerating shortly after the tetrad stage.

MISCELLANEOUS: In cv. C24, the gene is expressed in the young ovular primordia, but the protein is not present in these organs.

UniProt features for EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1
SIGNAL 1 18 Potential.
CHAIN 19 1192 Leucine-rich repeat receptor protein kinase EXS.
REPEAT 64 87 LRR 1.
REPEAT 90 112 LRR 2.
REPEAT 114 137 LRR 3.
REPEAT 138 160 LRR 4.
REPEAT 163 185 LRR 5.
REPEAT 187 209 LRR 6.
REPEAT 235 257 LRR 7.
REPEAT 259 281 LRR 8.
REPEAT 283 304 LRR 9.
REPEAT 330 352 LRR 10.
REPEAT 354 376 LRR 11.
REPEAT 378 400 LRR 12.
REPEAT 402 425 LRR 13.
REPEAT 426 447 LRR 14.
REPEAT 449 471 LRR 15.
REPEAT 473 496 LRR 16.
REPEAT 497 520 LRR 17.
REPEAT 521 543 LRR 18.
REPEAT 545 567 LRR 19.
REPEAT 581 603 LRR 20.
REPEAT 605 628 LRR 21.
REPEAT 629 651 LRR 22.
REPEAT 653 675 LRR 23.
REPEAT 677 697 LRR 24.
REPEAT 701 723 LRR 25.
REPEAT 725 748 LRR 26.
REPEAT 749 772 LRR 27.
REPEAT 773 795 LRR 28.
DOMAIN 917 1192 Protein kinase.
ACT_SITE 1043 1043 Proton acceptor (By similarity).
Amino Acid Sequence for EXS_ARATH » Leucine-rich repeat receptor protein kinase EMS1
MAFLTALFLF LFFSFSSSAI VDLSSETTSL ISFKRSLENP SLLSSWNVSS SASHCDWVGV TCLLGRVNSL SLPSLSLRGQ IPKEISSLKN LRELCLAGNQ FSGKIPPEIW NLKHLQTLDL SGNSLTGLLP RLLSELPQLL YLDLSDNHFS GSLPPSFFIS LPALSSLDVS NNSLSGEIPP EIGKLSNLSN LYMGLNSFSG QIPSEIGNIS LLKNFAAPSC FFNGPLPKEI SKLKHLAKLD LSYNPLKCSI PKSFGELHNL SILNLVSAEL IGLIPPELGN CKSLKSLMLS FNSLSGPLPL ELSEIPLLTF SAERNQLSGS LPSWMGKWKV LDSLLLANNR FSGEIPHEIE DCPMLKHLSL ASNLLSGSIP RELCGSGSLE AIDLSGNLLS GTIEEVFDGC SSLGELLLTN NQINGSIPED LWKLPLMALD LDSNNFTGEI PKSLWKSTNL MEFTASYNRL EGYLPAEIGN AASLKRLVLS DNQLTGEIPR EIGKLTSLSV LNLNANMFQG KIPVELGDCT SLTTLDLGSN NLQGQIPDKI TALAQLQCLV LSYNNLSGSI PSKPSAYFHQ IEMPDLSFLQ HHGIFDLSYN RLSGPIPEEL GECLVLVEIS LSNNHLSGEI PASLSRLTNL TILDLSGNAL TGSIPKEMGN SLKLQGLNLA NNQLNGHIPE SFGLLGSLVK LNLTKNKLDG PVPASLGNLK ELTHMDLSFN NLSGELSSEL STMEKLVGLY IEQNKFTGEI PSELGNLTQL EYLDVSENLL SGEIPTKICG LPNLEFLNLA KNNLRGEVPS DGVCQDPSKA LLSGNKELCG RVVGSDCKIE GTKLRSAWGI AGLMLGFTII VFVFVFSLRR WAMTKRVKQR DDPERMEESR LKGFVDQNLY FLSGSRSREP LSINIAMFEQ PLLKVRLGDI VEATDHFSKK NIIGDGGFGT VYKACLPGEK TVAVKKLSEA KTQGNREFMA EMETLGKVKH PNLVSLLGYC SFSEEKLLVY EYMVNGSLDH WLRNQTGMLE VLDWSKRLKI AVGAARGLAF LHHGFIPHII HRDIKASNIL LDGDFEPKVA DFGLARLISA CESHVSTVIA GTFGYIPPEY GQSARATTKG DVYSFGVILL ELVTGKEPTG PDFKESEGGN LVGWAIQKIN QGKAVDVIDP LLVSVALKNS QLRLLQIAML CLAETPAKRP NMLDVLKALK EI