EPHB1_HUMAN » Ephrin type-B receptor 1

EPHB1_HUMAN » Ephrin type-B receptor 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
EPHB1_HUMAN » Ephrin type-B receptor 1 » ELK;EPH tyrosine kinase 2;EPH-like kinase 6;EK6; hEK6; Neuronally-expressed EPH-related tyrosine kinase;NET; Tyrosine-protein kinase receptor EPH-2;
Hydrophobic Thickness 34.8 ± 1.4 Å
Tilt Angle 1 ± 0°
ΔGtransfer -39.4 kcal/mol
ΔGfold -15.1 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC, HMDB
Topology Out
TM Segments 538-563 (538-567)
Pathways

Axon guidance (KEGG)

PDB 2djs (434-528), 3zfx (602-896), 2eao (899-984)
OPM none
Complexes none
Interactions

EFNB1, Complex: EPHB1:EFNB1, PubMed

EFNB2, Complex: EFNB2:EPHB1, PubMed

EPHB6, Complex: EPHB1:EPHB6, PubMed

PGFRB, Complex: PGFRB:EPHB1, PubMed

Domains

AA: 20-196, PDBID: 1KGY, Subunit A, Seq Identity:77%, Ephrin receptor ligand binding domain

AA: 324-418, PDBID: 4BK4, Subunit A, Seq Identity:45%, Fibronectin type III domain

AA: 433-518, PDBID: 2DJS, Subunit A, Seq Identity:100%, Fibronectin type III domain

AA: 542-616, PDBID: 3ZFX, Subunit G, Seq Identity:100%, Ephrin type-A receptor 2 transmembrane domain

AA: 619-878, PDBID: 3ZFX, Subunit I, Seq Identity:100%, Protein tyrosine kinase

AA: 909-973, PDBID: 2EAO, Subunit A, Seq Identity:100%, SAM domain (Sterile alpha motif)

UniProt annotation for EPHB1_HUMAN » Ephrin type-B receptor 1
FUNCTION: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

SUBUNIT: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity). Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1 (By similarity). Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion (By similarity). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.

TISSUE SPECIFICITY: Preferentially expressed in brain.

UniProt features for EPHB1_HUMAN » Ephrin type-B receptor 1
SIGNAL 1 17
CHAIN 18 984 Ephrin type-B receptor 1.
DOMAIN 19 201 Eph LBD.
DOMAIN 323 424 Fibronectin type-III 1.
DOMAIN 430 525 Fibronectin type-III 2.
DOMAIN 619 882 Protein kinase.
DOMAIN 911 975 SAM.
MOTIF 982 984 PDZ-binding (Potential).
ACT_SITE 744 744 Proton acceptor (By similarity).
Amino Acid Sequence for EPHB1_HUMAN » Ephrin type-B receptor 1
MALDYLLLLL LASAVAAMEE TLMDTRTATA ELGWTANPAS GWEEVSGYDE NLNTIRTYQV CNVFEPNQNN WLLTTFINRR GAHRIYTEMR FTVRDCSSLP NVPGSCKETF NLYYYETDSV IATKKSAFWS EAPYLKVDTI AADESFSQVD FGGRLMKVNT EVRSFGPLTR NGFYLAFQDY GACMSLLSVR VFFKKCPSIV QNFAVFPETM TGAESTSLVI ARGTCIPNAE EVDVPIKLYC NGDGEWMVPI GRCTCKPGYE PENSVACKAC PAGTFKASQE AEGCSHCPSN SRSPAEASPI CTCRTGYYRA DFDPPEVACT SVPSGPRNVI SIVNETSIIL EWHPPRETGG RDDVTYNIIC KKCRADRRSC SRCDDNVEFV PRQLGLTECR VSISSLWAHT PYTFDIQAIN GVSSKSPFPP QHVSVNITTN QAAPSTVPIM HQVSATMRSI TLSWPQPEQP NGIILDYEIR YYEKEHNEFN SSMARSQTNT ARIDGLRPGM VYVVQVRART VAGYGKFSGK MCFQTLTDDD YKSELREQLP LIAGSAAAGV VFVVSLVAIS IVCSRKRAYS KEAVYSDKLQ HYSTGRGSPG MKIYIDPFTY EDPNEAVREF AKEIDVSFVK IEEVIGAGEF GEVYKGRLKL PGKREIYVAI KTLKAGYSEK QRRDFLSEAS IMGQFDHPNI IRLEGVVTKS RPVMIITEFM ENGALDSFLR QNDGQFTVIQ LVGMLRGIAA GMKYLAEMNY VHRDLAARNI LVNSNLVCKV SDFGLSRYLQ DDTSDPTYTS SLGGKIPVRW TAPEAIAYRK FTSASDVWSY GIVMWEVMSF GERPYWDMSN QDVINAIEQD YRLPPPMDCP AALHQLMLDC WQKDRNSRPR FAEIVNTLDK MIRNPASLKT VATITAVPSQ PLLDRSIPDF TAFTTVDDWL SAIKMVQYRD SFLTAGFTSL QLVTQMTSED LLRIGITLAG HQKKILNSIH SMRVQISQSP TAMA