EMP47_YEAST » Protein EMP47

EMP47_YEAST » Protein EMP47
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Topology in Golgi apparatus membrane
TopologyGolgi lumenal side
cytoplasmic side
EMP47_YEAST » Protein EMP47 » 47 kDa endomembrane protein;Endosomal P44 protein;
Hydrophobic Thickness 33.8 ± 3.3 Å
Tilt Angle 1 ± 3°
ΔGtransfer -27.5 kcal/mol
ΔGfold -24.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 410-432 (408-444)
Pathways none
PDB 2a6y (29-282), 2a6z (35-255), 2a70 (35-255), 2a71 (35-255)
OPM none
Complexes none
Interactions

ANP1, Complex: ANP1:EMP47, PubMed

ATG27, Complex: EMP47:ATG27, PubMed

EMP46, Complex: EMP46:EMP47, PubMed

ERP1, Complex: ERP1:EMP47, PubMed

ERP2, Complex: ERP2:EMP47, PubMed

HRD3, Complex: HRD1:UBX2:OS9:EMP47:USA1:CBPY:HRD3:CDC48:GRP78, PubMed

MKAR, Complex: MKAR:EMP47, PubMed

OST1, Complex: OST1:EMP47, PubMed

PMP2, Complex: EMP47:PMP2, PubMed

SEC22, Complex: SEC22:EMP47, PubMed

SED5, Complex: SED5:EMP47, PubMed

SRPB, Complex: SRPB:EMP47, PubMed

TMEDA, Complex: TMEDA:EMP47, PubMed

UBX2, Complex: HRD1:UBX2:OS9:EMP47:USA1:CBPY:HRD3:CDC48:GRP78, PubMed

Domains

AA: 36-253, PDBID: 2A6Y, Subunit A, Seq Identity:100%, Legume-like lectin family

UniProt annotation for EMP47_YEAST » Protein EMP47
FUNCTION: Involved in the secretion of glycoproteins and in nucleus architecture and gene silencing. Required for the endoplasmic reticulum exit of EMP46.

SUBUNIT: Homooligomers. Interacts with EMP46 in the endoplasmic reticulum membrane. Interacts with the coatomer proteins COP1, SEC21 and SEC23.

DOMAIN: The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.

MISCELLANEOUS: Present with 2900 molecules/cell in log phase SD medium.

UniProt features for EMP47_YEAST » Protein EMP47
SIGNAL 1 28
CHAIN 29 445 Protein EMP47.
DOMAIN 36 254 L-type lectin-like.
REGION 430 433 Mediates the interactions with COPI and COPII coat complexes (By similarity).
MOTIF 441 445 Di-lysine motif.
DISULFID 179 213
Amino Acid Sequence for EMP47_YEAST » Protein EMP47
MMMLITMKST VLLSVFTVLA TWAGLLEAHP LGDTSDASKL SSDYSLPDLI NARKVPNNWQ TGEQASLEEG RIVLTSKQNS KGSLWLKQGF DLKDSFTMEW TFRSVGYSGQ TDGGISFWFV QDSNVPRDKQ LYNGPVNYDG LQLLVDNNGP LGPTLRGQLN DGQKPVDKTK IYDQSFASCL MGYQDSSVPS TIRVTYDLED DNLLKVQVDN KVCFQTRKVR FPSGSYRIGV TAQNGAVNNN AESFEIFKMQ FFNGVIEDSL IPNVNAMGQP KLITKYIDQQ TGKEKLIEKT AFDADKDKIT NYELYKKLDR VEGKILANDI NALETKLNDV IKVQQELLSF MTTITKQLSS KPPANNEKGT STDDAIAEDK ENFKDFLSIN QKLEKVLVEQ EKYREATKRH GQDGPQVDEI ARKLMIWLLP LIFIMLVMAY YTFRIRQEII KTKLL