CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1

CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1 » Kinetochore-defect suppressor 4;
Hydrophobic Thickness 29.8 ± 3.0 Å
Tilt Angle 21 ± 6°
ΔGtransfer -26.0 kcal/mol
ΔGfold -12.8 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 3-23 (2-25)
Pathways

Protein processing in endoplasmic reticulum (KEGG)

PDB 4jqu (B=151-203)
OPM none
Complexes none
Interactions

CP51, Complex: CP51:CUE1, PubMed

EMC1, Complex: EMC1:CUE1

ERG2, Complex: ERG2:CUE1

GPI8, Complex: GPI8:CUE1

HLJ1, Complex: CUE1:HLJ1

HRD3, Complex: HRD3:CUE1

IRE1, Complex: IRE1:CUE1

MGA2, Complex: CUE1:MGA2

MMM1, Complex: CUE1:MMM1

MPS2, Complex: CUE1:MPS2

MSMO, Complex: MSMO:CUE1, PubMed

NEM1, Complex: NEM1:CUE1

OSTB, Complex: OSTB:CUE1, PubMed

PSD1, Complex: PSD1:CUE1

QCR8, Complex: QCR8:CUE1

TMEDA, Complex: TMEDA:CUE1

TOM6, Complex: CUE1:TOM6, PubMed

VOA1, Complex: CUE1:VOA1

YNS1, Complex: YNS1:CUE1

Domains

AA: 66-106, PDBID: 2EJS, Subunit A, Seq Identity:31%, CUE domain

UniProt annotation for CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1
FUNCTION: Component of the endoplasmic reticulum-associated protein degradation (ERAD) pathway. Recruits the soluble ubiquitin-conjugating enzyme UBC7 to the cytoplasmic face of the endoplasmic reticulum membrane where it functions in degradation of misfolded or regulated proteins localized in the endoplasmic reticulum (ER) lumen or membrane via the ubiquitin-proteasome system. Targets the E2 conjugating enzyme UBC7 to the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains, and to the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER- membrane proteins with misfolded transmembrane domains (ERAD-M). Has also a role in cold adaptation, perhaps through effects on sterol biosynthesis.

SUBUNIT: Forms a heterodimer with UBC7. Interacts with SSM4/DOA10 and UBX2/SEL1.

MISCELLANEOUS: Present with 589 molecules/cell in log phase SD medium.

UniProt features for CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1
CHAIN 1 203 Coupling of ubiquitin conjugation to ER degradation protein 1.
DOMAIN 65 107 CUE.
Amino Acid Sequence for CUE1_YEAST » Coupling of ubiquitin conjugation to ER degradation protein 1
MEDSRLLITL ILVFGVIFLK KFFQSNQHPS AQRLSATGVN AHGRPQGSTQ NALRRTGRVN GGHPVTTQMV ETVQNLAPNL HPEQIRYSLE NTGSVEETVE RYLRGDEFSF PPGFEPSRAP MGANAAVDNN AAGGGEFNDP RKKNMICAEN LLDKFHVDLN EDMSNLSFKD LDIEERKRLL VWQARKNLET KLQSDKDLQS LLT