CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4

CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4 » Chondroitin sulfate proteoglycan NG2;Melanoma chondroitin sulfate proteoglycan;Melanoma-associated chondroitin sulfate proteoglycan;
Hydrophobic Thickness 35.2 ± 2.6 Å
Tilt Angle 7 ± 9°
ΔGtransfer -34.5 kcal/mol
ΔGfold -23.4 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC, HMDB
Topology Out
TM Segments 2222-2246 (2219-2249)
Pathways none
PDB none
OPM none
Complexes none
Interactions

ITA3, Complex: CSPG4:ITA3, PubMed

ITB1, Complex: ITB1:CSPG4, PubMed

Domains

AA: 55-178, PDBID: 3SH4, Subunit A, Seq Identity:24%, Laminin G domain

AA: 230-363, PDBID: 3SH4, Subunit A, Seq Identity:22%, Laminin G domain

AA: 423-524, Cadherin-like

AA: 536-645, Cadherin-like

AA: 651-766, Cadherin-like

AA: 771-879, Cadherin-like

AA: 884-990, Cadherin-like

AA: 1001-1110, Cadherin-like

AA: 1115-1218, Cadherin-like

AA: 1221-1339, Cadherin-like

AA: 1358-1450, Cadherin-like

AA: 1456-1563, Cadherin-like

AA: 1572-1679, Cadherin-like

AA: 1691-1803, Cadherin-like

AA: 1815-1924, Cadherin-like

AA: 1930-2028, Cadherin-like

UniProt annotation for CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4
FUNCTION: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

TISSUE SPECIFICITY: Detected only in malignant melanoma cells.

MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.

UniProt features for CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4
SIGNAL 1 29 Potential.
CHAIN 30 2322 Chondroitin sulfate proteoglycan 4.
DOMAIN 30 192 Laminin G-like 1.
DOMAIN 202 380 Laminin G-like 2.
REPEAT 430 523 CSPG 1.
REPEAT 556 643 CSPG 2.
REPEAT 665 764 CSPG 3.
REPEAT 792 878 CSPG 4.
REPEAT 900 989 CSPG 5.
REPEAT 1020 1108 CSPG 6.
REPEAT 1128 1216 CSPG 7.
REPEAT 1241 1337 CSPG 8.
REPEAT 1360 1449 CSPG 9.
REPEAT 1476 1561 CSPG 10.
REPEAT 1583 1679 CSPG 11.
REPEAT 1707 1803 CSPG 12.
REPEAT 1835 1922 CSPG 13.
REPEAT 1944 2029 CSPG 14.
REPEAT 2048 2147 CSPG 15.
REGION 30 639 Globular or compact configuration stabilized by disulfide bonds.
REGION 30 639 Neurite growth inhibition (By similarity).
REGION 574 1040 Interaction with COL6A2 (By similarity).
REGION 631 1446 Interaction with COL5A1 (By similarity).
REGION 1586 2221 Neurite growth inhibition (By similarity).
REGION 1587 2221 Cysteine-containing.
MOTIF 2320 2322 PDZ-binding.
DISULFID 169 192 By similarity.
DISULFID 354 380 By similarity.
Amino Acid Sequence for CSPG4_HUMAN » Chondroitin sulfate proteoglycan 4
MQSGRGPPLP APGLALALTL TMLARLASAA SFFGENHLEV PVATALTDID LQLQFSTSQP EALLLLAAGP ADHLLLQLYS GRLQVRLVLG QEELRLQTPA ETLLSDSIPH TVVLTVVEGW ATLSVDGFLN ASSAVPGAPL EVPYGLFVGG TGTLGLPYLR GTSRPLRGCL HAATLNGRSL LRPLTPDVHE GCAEEFSASD DVALGFSGPH SLAAFPAWGT QDEGTLEFTL TTQSRQAPLA FQAGGRRGDF IYVDIFEGHL RAVVEKGQGT VLLHNSVPVA DGQPHEVSVH INAHRLEISV DQYPTHTSNR GVLSYLEPRG SLLLGGLDAE ASRHLQEHRL GLTPEATNAS LLGCMEDLSV NGQRRGLREA LLTRNMAAGC RLEEEEYEDD AYGHYEAFST LAPEAWPAME LPEPCVPEPG LPPVFANFTQ LLTISPLVVA EGGTAWLEWR HVQPTLDLME AELRKSQVLF SVTRGARHGE LELDIPGAQA RKMFTLLDVV NRKARFIHDG SEDTSDQLVL EVSVTARVPM PSCLRRGQTY LLPIQVNPVN DPPHIIFPHG SLMVILEHTQ KPLGPEVFQA YDPDSACEGL TFQVLGTSSG LPVERRDQPG EPATEFSCRE LEAGSLVYVH RGGPAQDLTF RVSDGLQASP PATLKVVAIR PAIQIHRSTG LRLAQGSAMP ILPANLSVET NAVGQDVSVL FRVTGALQFG ELQKQGAGGV EGAEWWATQA FHQRDVEQGR VRYLSTDPQH HAYDTVENLA LEVQVGQEIL SNLSFPVTIQ RATVWMLRLE PLHTQNTQQE TLTTAHLEAT LEEAGPSPPT FHYEVVQAPR KGNLQLQGTR LSDGQGFTQD DIQAGRVTYG ATARASEAVE DTFRFRVTAP PYFSPLYTFP IHIGGDPDAP VLTNVLLVVP EGGEGVLSAD HLFVKSLNSA SYLYEVMERP RHGRLAWRGT QDKTTMVTSF TNEDLLRGRL VYQHDDSETT EDDIPFVATR QGESSGDMAW EEVRGVFRVA IQPVNDHAPV QTISRIFHVA RGGRRLLTTD DVAFSDADSG FADAQLVLTR KDLLFGSIVA VDEPTRPIYR FTQEDLRKRR VLFVHSGADR GWIQLQVSDG QHQATALLEV QASEPYLRVA NGSSLVVPQG GQGTIDTAVL HLDTNLDIRS GDEVHYHVTA GPRWGQLVRA GQPATAFSQQ DLLDGAVLYS HNGSLSPRDT MAFSVEAGPV HTDATLQVTI ALEGPLAPLK LVRHKKIYVF QGEAAEIRRD QLEAAQEAVP PADIVFSVKS PPSAGYLVMV SRGALADEPP SLDPVQSFSQ EAVDTGRVLY LHSRPEAWSD AFSLDVASGL GAPLEGVLVE LEVLPAAIPL EAQNFSVPEG GSLTLAPPLL RVSGPYFPTL LGLSLQVLEP PQHGALQKED GPQARTLSAF SWRMVEEQLI RYVHDGSETL TDSFVLMANA SEMDRQSHPV AFTVTVLPVN DQPPILTTNT GLQMWEGATA PIPAEALRST DGDSGSEDLV YTIEQPSNGR VVLRGAPGTE VRSFTQAQLD GGLVLFSHRG TLDGGFRFRL SDGEHTSPGH FFRVTAQKQV LLSLKGSQTL TVCPGSVQPL SSQTLRASSS AGTDPQLLLY RVVRGPQLGR LFHAQQDSTG EALVNFTQAE VYAGNILYEH EMPPEPFWEA HDTLELQLSS PPARDVAATL AVAVSFEAAC PQRPSHLWKN KGLWVPEGQR ARITVAALDA SNLLASVPSP QRSEHDVLFQ VTQFPSRGQL LVSEEPLHAG QPHFLQSQLA AGQLVYAHGG GGTQQDGFHF RAHLQGPAGA SVAGPQTSEA FAITVRDVNE RPPQPQASVP LRLTRGSRAP ISRAQLSVVD PDSAPGEIEY EVQRAPHNGF LSLVGGGLGP VTRFTQADVD SGRLAFVANG SSVAGIFQLS MSDGASPPLP MSLAVDILPS AIEVQLRAPL EVPQALGRSS LSQQQLRVVS DREEPEAAYR LIQGPQYGHL LVGGRPTSAF SQFQIDQGEV VFAFTNFSSS HDHFRVLALA RGVNASAVVN VTVRALLHVW AGGPWPQGAT LRLDPTVLDA GELANRTGSV PRFRLLEGPR HGRVVRVPRA RTEPGGSQLV EQFTQQDLED GRLGLEVGRP EGRAPGPAGD SLTLELWAQG VPPAVASLDF ATEPYNAARP YSVALLSVPE AARTEAGKPE SSTPTGEPGP MASSPEPAVA KGGFLSFLEA NMFSVIIPMC LVLLLLALIL PLLFYLRKRN KTGKHDVQVL TAKPRNGLAG DTETFRKVEP GQAIPLTAVP GQGPPPGGQP DPELLQFCRT PNPALKNGQY WV