CERK1_ARATH » Chitin elicitor receptor kinase 1

CERK1_ARATH » Chitin elicitor receptor kinase 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
CERK1_ARATH » Chitin elicitor receptor kinase 1 » AtCERK1; LysM domain receptor-like kinase 1;LysM RLK1; LysM-containing receptor-like kinase 1;
Hydrophobic Thickness 32.4 ± 2.2 Å
Tilt Angle 6 ± 5°
ΔGtransfer -49.2 kcal/mol
ΔGfold -28.2 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 232-257 (232-262)
Pathways

Plant-pathogen interaction (KEGG)

PDB 4eby (25-230), 4ebz (25-230)
OPM none
Complexes none
Interactions

C71BF, Complex: CERK1:C71BF

C98A3, Complex: CERK1:C98A3

TCMO, Complex: CERK1:TCMO

Domains

AA: 170-208, PDBID: 4EBY, Subunit A, Seq Identity:100%, LysM domain

AA: 322-590, PDBID: 1BYG, Subunit A, Seq Identity:45%, Protein tyrosine kinase

UniProt annotation for CERK1_ARATH » Chitin elicitor receptor kinase 1
FUNCTION: Lysin motif (LysM) receptor kinase that functions as a cell surface receptor in chitin elicitor (chitooligosaccharides) signaling leading to innate immunity toward both biotic and abiotic stresses (e.g. tolerance to salinity, heavy-metal stresses, and Botrytis cinerea infection). Recognizes microbe- derived N-acetylglucosamine (NAG)-containing ligands. Involved in the resistance to pathogenic fungi Alternaria brassicicola and Erysiphe cichoracearum, probably by sensing microbe-associated molecular patterns (MAMP) and pathogen-associated molecular patterns (PAMP). Plays an essential role in detecting peptidoglycans (e.g. PGNs) and restricting bacterial growth. Target of the bacterial type III effector E3-ligase protein hopAB2/avrPtoB of Pseudomonas syringae pv. tomato DC3000 that mediates ubiquitination and subsequent proteolysis, thus blocking all defense responses by suppressing PAMP-triggered immunity (PTI).

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

ENZYME REGULATION: Activated by chitin-mediated homodimerization.

SUBUNIT: Forms homodimers and homooligomers. Homodimerization is required to trigger plant defenses. Binds to chitin, chitosan and chito-oligomer oligosaccharide elicitors. Interaction with chitin octamer (NAG(8)) promotes homodimerization while shorter chitin oligomers inhibit homodimerization. Interacts with Pseudomonas syringae hopAB2/avrPtoB.

TISSUE SPECIFICITY: Expressed ubiquitously, with lowest expression in pollen.

INDUCTION: Induced upon treatment with chitin oligosaccharide elicitor and flagellin (e.g. flg22).

UniProt features for CERK1_ARATH » Chitin elicitor receptor kinase 1
SIGNAL 1 23 Potential.
CHAIN 24 617 Chitin elicitor receptor kinase 1.
REPEAT 46 74 LysM 1; degenerate.
REPEAT 108 140 LysM 2; degenerate.
REPEAT 173 200 LysM 3.
DOMAIN 322 594 Protein kinase.
REGION 109 115 Chitin-binding.
REGION 137 143 Chitin-binding.
ACT_SITE 441 441 Proton acceptor (By similarity).
DISULFID 25 93
DISULFID 29 155
DISULFID 91 153
Amino Acid Sequence for CERK1_ARATH » Chitin elicitor receptor kinase 1
MKLKISLIAP ILLLFSFFFA VESKCRTSCP LALASYYLEN GTTLSVINQN LNSSIAPYDQ INFDPILRYN SNIKDKDRIQ MGSRVLVPFP CECQPGDFLG HNFSYSVRQE DTYERVAISN YANLTTMESL QARNPFPATN IPLSATLNVL VNCSCGDESV SKDFGLFVTY PLRPEDSLSS IARSSGVSAD ILQRYNPGVN FNSGNGIVYV PGRDPNGAFP PFKSSKQDGV GAGVIAGIVI GVIVALLLIL FIVYYAYRKN KSKGDSFSSS IPLSTKADHA SSTSLQSGGL GGAGVSPGIA AISVDKSVEF SLEELAKATD NFNLSFKIGQ GGFGAVYYAE LRGEKAAIKK MDMEASKQFL AELKVLTRVH HVNLVRLIGY CVEGSLFLVY EYVENGNLGQ HLHGSGREPL PWTKRVQIAL DSARGLEYIH EHTVPVYVHR DIKSANILID QKFRAKVADF GLTKLTEVGG SATRGAMGTF GYMAPETVYG EVSAKVDVYA FGVVLYELIS AKGAVVKMTE AVGEFRGLVG VFEESFKETD KEEALRKIID PRLGDSYPFD SVYKMAELGK ACTQENAQLR PSMRYIVVAL STLFSSTGNW DVGNFQNEDL VSLMSGR