C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase

C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase » Cytochrome P450 90D1;
Hydrophobic Thickness 28.6 ± 1.8 Å
Tilt Angle 6 ± 2°
ΔGtransfer -24.4 kcal/mol
ΔGfold -17.5 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 5-24 (3-24)
Pathways

Biosynthesis of secondary metabolites (KEGG)

Brassinosteroid biosynthesis (KEGG)

brassinosteroid biosynthesis I (BioCyc)

brassinosteroid biosynthesis II (BioCyc)

PDB none
OPM none
Complexes none
Interactions none
Domains

AA: 52-488, PDBID: 2VE3, Subunit A, Seq Identity:28%, Cytochrome P450

UniProt annotation for C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase
FUNCTION: C-23 hydroxylase involved in brassinosteroids biosynthesis. Converts directly (22S,24R)-22-hydroxy-5-alpha- ergostan-3-one and 3-epi-6-deoxocathasterone to 3-dehydro-6- deoxoteasterone and 6-deoxotyphasterol. These C-23 hydroxylation shortcuts bypass campestanol, 6-deoxocathasterone, and 6- deoxoteasterone. Functionally redundant with CYP90C1.

CATALYTIC ACTIVITY: 3-epi-6-deoxocathasterone + NADPH + O(2) = 6- deoxotyphasterol + NADP(+) + H(2)O.

CATALYTIC ACTIVITY: (22S,24R)-22-hydroxy-5-alpha-ergostan-3-one + NADPH + O(2) = 3-dehydro-6-deoxoteasterone + NADP(+) + H(2)O.

TISSUE SPECIFICITY: Expressed in leaf vascular tissue.

UniProt features for C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase
CHAIN 1 491 3-epi-6-deoxocathasterone 23- monooxygenase.
Amino Acid Sequence for C90D1_ARATH » 3-epi-6-deoxocathasterone 23-monooxygenase
MDTSSSLLFF SFFFFIIIVI FNKINGLRSS PASKKKLNDH HVTSQSHGPK FPHGSLGWPV IGETIEFVSS AYSDRPESFM DKRRLMYGRV FKSHIFGTAT IVSTDAEVNR AVLQSDSTAF VPFYPKTVRE LMGKSSILLI NGSLHRRFHG LVGSFLKSPL LKAQIVRDMH KFLSESMDLW SEDQPVLLQD VSKTVAFKVL AKALISVEKG EDLEELKREF ENFISGLMSL PINFPGTQLH RSLQAKKNMV KQVERIIEGK IRKTKNKEED DVIAKDVVDV LLKDSSEHLT HNLIANNMID MMIPGHDSVP VLITLAVKFL SDSPAALNLL TEENMKLKSL KELTGEPLYW NDYLSLPFTQ KVITETLRMG NVIIGVMRKA MKDVEIKGYV IPKGWCFLAY LRSVHLDKLY YESPYKFNPW RWQERDMNTS SFSPFGGGQR LCPGLDLARL ETSVFLHHLV TRFRWIAEED TIINFPTVHM KNKLPIWIKR I