BRL3_ARATH » Receptor-like protein kinase BRI1-like 3

BRL3_ARATH » Receptor-like protein kinase BRI1-like 3
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
BRL3_ARATH » Receptor-like protein kinase BRI1-like 3 » BRASSINOSTEROID INSENSITIVE 1-like protein 3;
Hydrophobic Thickness 30.0 ± 1.2 Å
Tilt Angle 0 ± 3°
ΔGtransfer -42.0 kcal/mol
ΔGfold -18.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 774-798 (771-800)
Pathways none
PDB none
OPM none
Complexes none
Interactions

BAK1, Complex: BAK1:BRL3

BRL1, Complex: BRL1:BRL3

FERON, Complex: BRL3:FERON

Domains

AA: 31-73, PDBID: 4J0M, Subunit B, Seq Identity:61%, Leucine rich repeat N-terminal domain

AA: 303-325, PDBID: 4MN8, Subunit A, Seq Identity:52%, Leucine Rich Repeat

AA: 376-398, PDBID: 4MN8, Subunit A, Seq Identity:54%, Leucine Rich Repeat

AA: 402-462, PDBID: 3RGX, Subunit A, Seq Identity:41%, Leucine rich repeat

AA: 664-723, PDBID: 4J0M, Subunit A, Seq Identity:90%, Leucine rich repeat

AA: 858-1132, PDBID: 4OA2, Subunit A, Seq Identity:76%, Protein kinase domain

UniProt annotation for BRL3_ARATH » Receptor-like protein kinase BRI1-like 3
FUNCTION: Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Binds brassinolide. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development. May be involved in cell growth and vascular differentiation.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

TISSUE SPECIFICITY: Predominantly expressed in vascular tissues. Expressed only during postembryonic development with a very discrete pattern of expression, preferentially in the two protophloem cell files at the elongation zone of the root. The expression in these two cell files attenuates as the phloem cells differentiate in the upper root. In cotyledons and leaves, it is expressed in phloem cells, starting at the cotyledons and shoot apex, moving toward the basal part of the leaves, where the expression is weak. Expressed in the secondary and tertiary veins and in the upper part of the cotyledons and leaves. Weakly or not expressed in the inflorescence stems. Has some complementary expression with BRL1.

DOMAIN: Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.

DOMAIN: A 70 amino acid island between the 18th and the 19th LRR is essential for the binding of brassinosteroids.

UniProt features for BRL3_ARATH » Receptor-like protein kinase BRI1-like 3
SIGNAL 1 23 Potential.
CHAIN 24 1164 Receptor-like protein kinase BRI1-like 3.
REPEAT 77 98 LRR 1.
REPEAT 102 123 LRR 2.
REPEAT 125 146 LRR 3.
REPEAT 151 173 LRR 4.
REPEAT 176 197 LRR 5.
REPEAT 202 224 LRR 6.
REPEAT 227 248 LRR 7.
REPEAT 252 274 LRR 8.
REPEAT 278 300 LRR 9.
REPEAT 303 325 LRR 10.
REPEAT 327 347 LRR 11.
REPEAT 352 375 LRR 12.
REPEAT 376 397 LRR 13.
REPEAT 403 424 LRR 14.
REPEAT 427 448 LRR 15.
REPEAT 451 473 LRR 16.
REPEAT 476 498 LRR 17.
REPEAT 500 523 LRR 18.
REPEAT 524 546 LRR 19.
REPEAT 548 570 LRR 20.
REPEAT 640 662 LRR 21.
REPEAT 664 686 LRR 22.
REPEAT 688 711 LRR 23.
REPEAT 712 734 LRR 24.
DOMAIN 858 1136 Protein kinase.
MOTIF 65 72 Cys pair 1.
MOTIF 748 755 Cys pair 2.
ACT_SITE 985 985 Proton acceptor (By similarity).
Amino Acid Sequence for BRL3_ARATH » Receptor-like protein kinase BRI1-like 3
MKQQWQFLIL CLLVLFLTVD SRGRRLLSDD VNDTALLTAF KQTSIKSDPT NFLGNWRYGS GRDPCTWRGV SCSSDGRVIG LDLRNGGLTG TLNLNNLTAL SNLRSLYLQG NNFSSGDSSS SSGCSLEVLD LSSNSLTDSS IVDYVFSTCL NLVSVNFSHN KLAGKLKSSP SASNKRITTV DLSNNRFSDE IPETFIADFP NSLKHLDLSG NNVTGDFSRL SFGLCENLTV FSLSQNSISG DRFPVSLSNC KLLETLNLSR NSLIGKIPGD DYWGNFQNLR QLSLAHNLYS GEIPPELSLL CRTLEVLDLS GNSLTGQLPQ SFTSCGSLQS LNLGNNKLSG DFLSTVVSKL SRITNLYLPF NNISGSVPIS LTNCSNLRVL DLSSNEFTGE VPSGFCSLQS SSVLEKLLIA NNYLSGTVPV ELGKCKSLKT IDLSFNALTG LIPKEIWTLP KLSDLVMWAN NLTGGIPESI CVDGGNLETL ILNNNLLTGS LPESISKCTN MLWISLSSNL LTGEIPVGIG KLEKLAILQL GNNSLTGNIP SELGNCKNLI WLDLNSNNLT GNLPGELASQ AGLVMPGSVS GKQFAFVRNE GGTDCRGAGG LVEFEGIRAE RLEHFPMVHS CPKTRIYSGM TMYMFSSNGS MIYLDLSYNA VSGSIPLGYG AMGYLQVLNL GHNLLTGTIP DSFGGLKAIG VLDLSHNDLQ GFLPGSLGGL SFLSDLDVSN NNLTGPIPFG GQLTTFPLTR YANNSGLCGV PLPPCSSGSR PTRSHAHPKK QSIATGMSAG IVFSFMCIVM LIMALYRARK VQKKEKQREK YIESLPTSGS SSWKLSSVHE PLSINVATFE KPLRKLTFAH LLEATNGFSA DSMIGSGGFG DVYKAKLADG SVVAIKKLIQ VTGQGDREFM AEMETIGKIK HRNLVPLLGY CKIGEERLLV YEYMKYGSLE TVLHEKTKKG GIFLDWSARK KIAIGAARGL AFLHHSCIPH IIHRDMKSSN VLLDQDFVAR VSDFGMARLV SALDTHLSVS TLAGTPGYVP PEYYQSFRCT AKGDVYSYGV ILLELLSGKK PIDPEEFGED NNLVGWAKQL YREKRGAEIL DPELVTDKSG DVELLHYLKI ASQCLDDRPF KRPTMIQVMT MFKELVQVDT ENDSLDEFLL KETPLVEESR DKEP