BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1

BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1 » BRASSINOSTEROID INSENSITIVE 1-like protein 1;
Hydrophobic Thickness 41.2 ± 4.2 Å
Tilt Angle 1 ± 4°
ΔGtransfer -29.0 kcal/mol
ΔGfold -22.7 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 771-799 (767-801)
Pathways none
PDB 4j0m (25-758)
OPM none
Complexes none
Interactions

BAK1, Complex: BAK1:BRL1

BRL3, Complex: BRL1:BRL3

Domains

AA: 33-74, PDBID: 4J0M, Subunit B, Seq Identity:100%, Leucine rich repeat N-terminal domain

AA: 123-141, PDBID: 4J0M, Subunit B, Seq Identity:100%, Leucine Rich repeat

AA: 200-216, PDBID: 4J0M, Subunit B, Seq Identity:100%, Leucine Rich repeat

AA: 373-390, PDBID: 4J0M, Subunit B, Seq Identity:100%, Leucine Rich repeat

AA: 424-442, PDBID: 4J0M, Subunit B, Seq Identity:100%, Leucine Rich repeat

AA: 663-723, PDBID: 4J0M, Subunit A, Seq Identity:100%, Leucine rich repeat

AA: 859-1134, PDBID: 4OA2, Subunit A, Seq Identity:67%, Protein kinase domain

UniProt annotation for BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1
FUNCTION: Receptor with a serine/threonine-protein kinase activity. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development. Binds brassinolide. May be involved in cell growth and vascular differentiation.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

TISSUE SPECIFICITY: Predominantly expressed in vascular tissues. From 7 day old seedlings, it is expressed in the columella cells of the root tip, in the vascular initials in the meristematic region of the root and in vascular tissues. After germination, it is expressed in the stele cell and in the early differentiation zone of the root, where the expression continues from the root to the hypocotyls and cotyledons following the midvein. In mature plants, it is expressed in the vasculature of the leaf, predominantly in the midvein, and in the vascular bundles of inflorescence stems. Localizes to procambial cells of the vascular bundles located between the differentiating xylem and the phloem.

DOMAIN: Contains two pairs of conservatively spaced Cys (Cys pair 1 and 2) possibly involved in forming some heterodimers.

DOMAIN: A 70 amino acid island between the 19th and the 20th LRR is essential for the binding of brassinosteroids.

UniProt features for BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1
SIGNAL 1 21 Potential.
CHAIN 22 1166 Serine/threonine-protein kinase BRI1-like 1.
REPEAT 78 99 LRR 1.
REPEAT 103 124 LRR 2.
REPEAT 126 147 LRR 3.
REPEAT 152 173 LRR 4.
REPEAT 176 197 LRR 5.
REPEAT 202 224 LRR 6.
REPEAT 227 248 LRR 7.
REPEAT 252 274 LRR 8.
REPEAT 278 300 LRR 9.
REPEAT 303 325 LRR 10.
REPEAT 327 349 LRR 11.
REPEAT 352 375 LRR 12.
REPEAT 376 397 LRR 13.
REPEAT 403 424 LRR 14.
REPEAT 427 449 LRR 15.
REPEAT 451 473 LRR 16.
REPEAT 476 498 LRR 17.
REPEAT 500 522 LRR 18.
REPEAT 524 547 LRR 19.
REPEAT 548 570 LRR 20.
REPEAT 664 686 LRR 21.
REPEAT 688 710 LRR 22.
REPEAT 712 734 LRR 23.
DOMAIN 859 1147 Protein kinase.
MOTIF 66 73 Cys pair 1.
MOTIF 748 755 Cys pair 2.
ACT_SITE 987 987 Proton acceptor (By similarity).
Amino Acid Sequence for BRL1_ARATH » Serine/threonine-protein kinase BRI1-like 1
MKQRWLLVLI LCFFTTSLVM GIHGKHLIND DFNETALLLA FKQNSVKSDP NNVLGNWKYE SGRGSCSWRG VSCSDDGRIV GLDLRNSGLT GTLNLVNLTA LPNLQNLYLQ GNYFSSGGDS SGSDCYLQVL DLSSNSISDY SMVDYVFSKC SNLVSVNISN NKLVGKLGFA PSSLQSLTTV DLSYNILSDK IPESFISDFP ASLKYLDLTH NNLSGDFSDL SFGICGNLTF FSLSQNNLSG DKFPITLPNC KFLETLNISR NNLAGKIPNG EYWGSFQNLK QLSLAHNRLS GEIPPELSLL CKTLVILDLS GNTFSGELPS QFTACVWLQN LNLGNNYLSG DFLNTVVSKI TGITYLYVAY NNISGSVPIS LTNCSNLRVL DLSSNGFTGN VPSGFCSLQS SPVLEKILIA NNYLSGTVPM ELGKCKSLKT IDLSFNELTG PIPKEIWMLP NLSDLVMWAN NLTGTIPEGV CVKGGNLETL ILNNNLLTGS IPESISRCTN MIWISLSSNR LTGKIPSGIG NLSKLAILQL GNNSLSGNVP RQLGNCKSLI WLDLNSNNLT GDLPGELASQ AGLVMPGSVS GKQFAFVRNE GGTDCRGAGG LVEFEGIRAE RLERLPMVHS CPATRIYSGM TMYTFSANGS MIYFDISYNA VSGFIPPGYG NMGYLQVLNL GHNRITGTIP DSFGGLKAIG VLDLSHNNLQ GYLPGSLGSL SFLSDLDVSN NNLTGPIPFG GQLTTFPVSR YANNSGLCGV PLRPCGSAPR RPITSRIHAK KQTVATAVIA GIAFSFMCFV MLVMALYRVR KVQKKEQKRE KYIESLPTSG SCSWKLSSVP EPLSINVATF EKPLRKLTFA HLLEATNGFS AETMVGSGGF GEVYKAQLRD GSVVAIKKLI RITGQGDREF MAEMETIGKI KHRNLVPLLG YCKVGEERLL VYEYMKWGSL ETVLHEKSSK KGGIYLNWAA RKKIAIGAAR GLAFLHHSCI PHIIHRDMKS SNVLLDEDFE ARVSDFGMAR LVSALDTHLS VSTLAGTPGY VPPEYYQSFR CTAKGDVYSY GVILLELLSG KKPIDPGEFG EDNNLVGWAK QLYREKRGAE ILDPELVTDK SGDVELFHYL KIASQCLDDR PFKRPTMIQL MAMFKEMKAD TEEDESLDEF SLKETPLVEE SRDKEP