BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1

BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 » AtBAK1; BRI1-associated receptor kinase 1; Protein ELONGATED;Somatic embryogenesis receptor kinase 3;AtSERK3; Somatic embryogenesis receptor-like kinase 3;
Hydrophobic Thickness 34.4 ± 2.2 Å
Tilt Angle 2 ± 0°
ΔGtransfer -18.5 kcal/mol
ΔGfold -18.1 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 227-249 (217-253)
Pathways

Plant hormone signal transduction (KEGG)

Plant-pathogen interaction (KEGG)

Protein processing in endoplasmic reticulum (KEGG)

Viral carcinogenesis (KEGG)

PDB 3uim (258-583), 3tl8 (A/D/G/H=250-590), 3ulz (A=258-583), 4mn8 (B=1-220), 4m7e (C/D=26-220)
OPM none
Complexes

BAK1:FLS2_ARATH

BAK1:BRI1_ARATH

Interactions

BRI1, Complex: BAK1:BRI1, PDBID: 4M7E, PubMed

BRL1, Complex: BAK1:BRL1

BRL3, Complex: BAK1:BRL3

EFR, Complex: BAK1:EFR

FLS2, Complex: FLS2:BAK1, PDBID: 4MN8, PubMed

P2C70, Complex: P2C70:BAK1, PubMed

PEPR1, Complex: BAK1:PEPR1

PEPR2, Complex: BAK1:PEPR2

SERK1, Complex: BAK1:SERK1

Y5838, Complex: BAK1:Y5838, PubMed

Domains

AA: 25-65, PDBID: 4M7E, Subunit D, Seq Identity:100%, Leucine rich repeat N-terminal domain

AA: 92-152, PDBID: 4M7E, Subunit C, Seq Identity:100%, Leucine rich repeat

AA: 165-189, PDBID: 4M7E, Subunit C, Seq Identity:100%, Leucine Rich Repeat

AA: 289-563, PDBID: 3TL8, Subunit A, Seq Identity:100%, Protein kinase domain

UniProt annotation for BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
FUNCTION: Dual specificity kinase acting on both serine/threonine- and tyrosine-containing substrates. Controls the expression of genes associated with innate immunity in the absence of pathogens or elicitors. Involved in brassinosteroid (BR) signal transduction. Phosphorylates BRI1. May be involved in changing the equilibrium between plasma membrane-located BRI1 homodimers and endocytosed BRI1-BAK1 heterodimers. Interaction with MSBP1 stimulates the endocytosis of BAK1 and suppresses brassinosteroid signaling. Acts in pathogen-associated molecular pattern (PAMP)- triggered immunity (PTI) via its interactions with FLS2 and EFR, and the phosphorylation of BIK1. Involved in programmed cell death (PCD) control. Positively regulates the BR-dependent plant growth pathway and negatively regulates the BR-independent cell-death pathway.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

SUBUNIT: Monomer. Heterodimer with BRI1 in the endosomes. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1. Interacts with the P.syringae AvrPto and hopAB2/AvrPtoB, ERD13, PEPR1 and PEPR2. Interacts (via extracellular region) with MSBP1. Interacts with the EF-Tu receptor EFR and FLS2 in a specific ligand-induced manner.

TISSUE SPECIFICITY: Expressed ubiquitously.

INDUCTION: Up-regulated by flagellin and harpin.

DOMAIN: Contains one leucine-zipper motif and one pair of conservatively spaced Cys (Cys pair) involved in forming heterodimers.

MISCELLANEOUS: Interaction with BRI1 activates both receptor kinases and the full activation of either receptor kinase requires transphosphorylation by their partners. This interaction in vitro is magnesium dependent. Instantaneous heteromeric complex formation between FLS2 and BAK1 and reciprocal transphosphorylation after binding of the flagellin flg22 ligand to FLS2. The kinase activity is not required for the complex formation.

MISCELLANEOUS: The interaction with the bacterial effectors AvrPto and AvrPtoB/hopAB2 interfers with FLS2 binding and plant immunity.

UniProt features for BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
SIGNAL 1 25 Potential.
CHAIN 26 615 BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1.
REPEAT 93 116 LRR 1.
REPEAT 117 140 LRR 2.
REPEAT 141 163 LRR 3.
REPEAT 165 187 LRR 4.
DOMAIN 289 576 Protein kinase.
MOTIF 57 64 Cys pair.
ACT_SITE 416 416 Proton acceptor (By similarity).
Amino Acid Sequence for BAK1_ARATH » BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1
MERRLMIPCF FWLILVLDLV LRVSGNAEGD ALSALKNSLA DPNKVLQSWD ATLVTPCTWF HVTCNSDNSV TRVDLGNANL SGQLVMQLGQ LPNLQYLELY SNNITGTIPE QLGNLTELVS LDLYLNNLSG PIPSTLGRLK KLRFLRLNNN SLSGEIPRSL TAVLTLQVLD LSNNPLTGDI PVNGSFSLFT PISFANTKLT PLPASPPPPI SPTPPSPAGS NRITGAIAGG VAAGAALLFA VPAIALAWWR RKKPQDHFFD VPAEEDPEVH LGQLKRFSLR ELQVASDNFS NKNILGRGGF GKVYKGRLAD GTLVAVKRLK EERTQGGELQ FQTEVEMISM AVHRNLLRLR GFCMTPTERL LVYPYMANGS VASCLRERPE SQPPLDWPKR QRIALGSARG LAYLHDHCDP KIIHRDVKAA NILLDEEFEA VVGDFGLAKL MDYKDTHVTT AVRGTIGHIA PEYLSTGKSS EKTDVFGYGV MLLELITGQR AFDLARLAND DDVMLLDWVK GLLKEKKLEA LVDVDLQGNY KDEEVEQLIQ VALLCTQSSP MERPKMSEVV RMLEGDGLAE RWEEWQKEEM FRQDFNYPTH HPAVSGWIIG DSTSQIENEY PSGPR