ANR27_HUMAN » Ankyrin repeat domain-containing protein 27

ANR27_HUMAN » Ankyrin repeat domain-containing protein 27
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
ANR27_HUMAN » Ankyrin repeat domain-containing protein 27 » VPS9 domain-containing protein;
Hydrophobic Thickness 33.6 ± 2.3 Å
Tilt Angle 3 ± 3°
ΔGtransfer -17.6 kcal/mol
ΔGfold -4.0 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING, HGNC
Topology Und
TM Segments 79-101 (76-104)
Pathways none
PDB 4b93 (B=659-921), 4cz2 (D/E/F=450-640), 4cym (D/E/F=450-640)
OPM none
Complexes none
Interactions none
Domains

AA: 264-367, PDBID: 2EFC, Subunit C, Seq Identity:30%, Vacuolar sorting protein 9 (VPS9) domain

AA: 401-526, PDBID: 4CYM, Subunit F, Seq Identity:100%, Ankyrin repeats (3 copies)

AA: 492-559, PDBID: 4CYM, Subunit F, Seq Identity:100%, Ankyrin repeats (3 copies)

AA: 520-595, PDBID: 4CYM, Subunit F, Seq Identity:100%, Ankyrin repeats (3 copies)

AA: 673-807, PDBID: 4B93, Subunit B, Seq Identity:100%, Ankyrin repeats (3 copies)

AA: 781-873, PDBID: 4B93, Subunit B, Seq Identity:100%, Ankyrin repeats (3 copies)

UniProt annotation for ANR27_HUMAN » Ankyrin repeat domain-containing protein 27
FUNCTION: May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (PubMed:16525121, PubMed:18477474). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (PubMed:23104059). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (By similarity). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (By similarity). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1 (PubMed:22705394). Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (PubMed:19745841). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (PubMed:24856514).
UniProt features for ANR27_HUMAN » Ankyrin repeat domain-containing protein 27
CHAIN 1 1050 Ankyrin repeat domain-containing protein 27.

DOMAIN 233 371 VPS9.

REPEAT 396 426 ANK 1.

REPEAT 462 491 ANK 2.

REPEAT 495 524 ANK 3.

REPEAT 528 560 ANK 4.

REPEAT 564 593 ANK 5.

REPEAT 597 627 ANK 6.

REPEAT 668 698 ANK 7.

REPEAT 743 772 ANK 8.

REPEAT 776 805 ANK 9.

REPEAT 809 838 ANK 10.

REPEAT 842 871 ANK 11.

REGION 1 372 Sufficient for GEF activity towards RAB21.

REGION 396 460 Sufficient for interaction with VPS29.

REGION 451 730 Interaction with RAB32.

REGION 451 600 Interaction with RAB38.

REGION 658 707 Required for interaction with VAMP7.

REGION 692 746 Sufficient for interaction with VPS29.

Amino Acid Sequence for ANR27_HUMAN » Ankyrin repeat domain-containing protein 27
MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST CQFESYILIP VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF YNEKEESFSI LCIAHPLEKR ESSEEPLAPS DPFSLKTIED VREFLGRHSE RFDRNIASFH RTFRECERKS LRHHIDSANA LYTKCLQQLL RDSHLKMLAK QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN KITRSLQDLQ QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSLAKDELGY CLTSFEAAIE YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC LFKHIASGNQ KEVERLLSQE DHDKDTVQKM CHPLCFCDDC EKLVSGRLND PSVVTPFSRD DRGHTPLHVA AVCGQASLID LLVSKGAMVN ATDYHGATPL HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG HEDCVKALVY YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS FSSMSASSRQ EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE DTVSAADPEF CHPLCQCPKC APAQKRLAKV PASGLGVNVT SQDGSSPLHV AALHGRADLI PLLLKHGANA GARNADQAVP LHLACQQGHF QVVKCLLDSN AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN NKGNTALHEA VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG KTSREIMARD RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS DWPERPGLTQ TGPGHRRMLR RHTVEDAVVS QGPEAAGPLS TPQEVSASRS