ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase

ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase
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Topology in Endoplasmic reticulum membrane
Topologylumenal side
cytoplasmic side
ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase » Asparagine-linked glycosylation protein 1;Beta-1,4-mannosyltransferase;GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase;GDP-mannose-dolichol diphosphochitobiose mannosyltransferase;
Hydrophobic Thickness 29.6 ± 1.8 Å
Tilt Angle 1 ± 0°
ΔGtransfer -34.1 kcal/mol
ΔGfold -13.9 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology In
TM Segments 4-30 (4-35)
Pathways

dolichyl-diphosphooligosaccharide biosynthesis (BioCyc)

N-Glycan biosynthesis (KEGG)

Various types of N-glycan biosynthesis (KEGG)

PDB none
OPM none
Complexes none
Interactions

ALG11, Complex: ALG11:ALG1, PubMed

ALG11, Complex: ALG11:ALG2:ALG1, PubMed

CY1, Complex: ALG1:CY1

EMP24, Complex: ALG1:EMP24, PubMed

FET3, Complex: ALG1:FET3, PubMed

FUS1, Complex: ALG1:FUS1, PubMed

GPI8, Complex: ALG1:GPI8, PubMed

MKAR, Complex: MKAR:ALG1, PubMed

MMM1, Complex: ALG1:MMM1

MSMO, Complex: MSMO:ALG1, PubMed

OSTB, Complex: OSTB:ALG1, PubMed

PHB2, Complex: ALG1:PHB2

PMP2, Complex: ALG1:PMP2, PubMed

SKN1, Complex: SKN1:ALG1

Domains

AA: 252-429, PDBID: 2F9F, Subunit A, Seq Identity:18%, Glycosyl transferases group 1

UniProt annotation for ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase
FUNCTION: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.

CATALYTIC ACTIVITY: GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.

SUBUNIT: Homodimer. Interacts with ALG2 and ALG11.

MISCELLANEOUS: Present with 1890 molecules/cell in log phase SD medium.

UniProt features for ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase
CHAIN 1 449 Chitobiosyldiphosphodolichol beta- mannosyltransferase.
REGION 435 449 Required for oligomerization.
MOTIF 21 32 Dolichol recognition (Potential).
Amino Acid Sequence for ALG1_YEAST » Chitobiosyldiphosphodolichol beta-mannosyltransferase
MFLEIPRWLL ALIILYLSIP LVVYYVIPYL FYGNKSTKKR IIIFVLGDVG HSPRICYHAI SFSKLGWQVE LCGYVEDTLP KIISSDPNIT VHHMSNLKRK GGGTSVIFMV KKVLFQVLSI FKLLWELRGS DYILVQNPPS IPILPIAVLY KLTGCKLIID WHNLAYSILQ LKFKGNFYHP LVLISYMVEM IFSKFADYNL TVTEAMRKYL IQSFHLNPKR CAVLYDRPAS QFQPLAGDIS RQKALTTKAF IKNYIRDDFD TEKGDKIIVT STSFTPDEDI GILLGALKIY ENSYVKFDSS LPKILCFITG KGPLKEKYMK QVEEYDWKRC QIEFVWLSAE DYPKLLQLCD YGVSLHTSSS GLDLPMKILD MFGSGLPVIA MNYPVLDELV QHNVNGLKFV DRRELHESLI FAMKDADLYQ KLKKNVTQEA ENRWQSNWER TMRDLKLIH