ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4

ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4
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Topology in Plasma membrane
Topologyextracellular side
cytoplasmic side
ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4 » Protein CRINKLY 4;AtCR4;
Hydrophobic Thickness 27.6 ± 2.4 Å
Tilt Angle 3 ± 1°
ΔGtransfer -39.4 kcal/mol
ΔGfold -18.6 kcal/mol
Links UniProtKB, Pfam, Interpro, iHOP, STRING
Topology Out
TM Segments 434-456 (434-460)
Pathways none
PDB none
OPM none
Complexes none
Interactions

CLV1, Complex: ACR4L:CLV1

Domains

AA: 170-199, PDBID: 3OF7, Subunit A, Seq Identity:24%, Regulator of chromosome condensation (RCC1) repeat

AA: 512-785, PDBID: 3TL8, Subunit A, Seq Identity:40%, Protein kinase domain

UniProt annotation for ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4
FUNCTION: Controls formative cell division in meristems, including root tips and lateral root initiation zones of the pericycle, in response to CLE40 signal. Acts with CLE40p peptide as a ligand- receptor pair in a signal transduction pathway, coordinating movement of the root tip and organization of cell divisions in the root meristem. Required during embryogenesis and development, probably for the differentiation of protoderm and epidermal cells. Involved in the regulation of cellular organization during the development of sepal margins and ovule integument outgrowth. Can phosphorylate ALE2.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

SUBUNIT: Homodimer.

TISSUE SPECIFICITY: Expressed in seedlings, floral buds, siliques, leaves, shoot apical meristems (SAM), and, to a lower extent, in roots.

DEVELOPMENTAL STAGE: First observed in all young embryo cells. At the globular stage, restricted to apical region. Later confined to the protoderm area leading to cotyledon primordia and the root apex. In mature embryos, mostly localized in the L1 layer of the SAM, apical regions of cotyledons and the root apex, and, to a lower extent, in protoderm of other regions. In seedlings, expressed in developing tissues of the shoot, including the SAM and epidermis of organs primordia, especially in L1 layer cells. In roots, localized in quiescent center (QC) central cells, columella initials and cells below the QC, the lateral root cap (LRC) and the initial cells destined to give rise to the root epidermal cell file and the LRC. Expressed in epidermal emerged from under the LRC, with levels vanishing in elongation zone. Specifically detected in the small daughter cells after the first asymmetric pericycle cell division during lateral roots emergence. Subsequently, the expression expands to the adjacent small daughter cells from the second asymmetric cell division, resulting in a central core-specific expression pattern.

INDUCTION: By CLE40 in root quiescent center (QC).

UniProt features for ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4
SIGNAL 1 29
CHAIN 30 895 Serine/threonine-protein kinase-like protein ACR4.
REPEAT 38 73 1.
REPEAT 77 112 2.
REPEAT 130 167 3.
REPEAT 169 202 4.
REPEAT 210 245 5.
REPEAT 262 296 6.
REPEAT 301 339 7.
REPEAT 346 395 TNFR-Cys.
DOMAIN 512 789 Protein kinase.
REGION 38 339 7 X 36 AA repeats.
ACT_SITE 641 641 Proton acceptor.
DISULFID 347 370
DISULFID 373 387
DISULFID 377 395
Amino Acid Sequence for ACR4L_ARATH » Serine/threonine-protein kinase-like protein ACR4
MRMFETRARE WILLVKLVLF TSIWQLASAL GSMSSIAISY GEGGSVFCGL KSDGSHLVVC YGSNSAILYG TPGHLQFIGL TGGDGFMCGL LMLSHQPYCW GNSAFIQMGV PQPMTKGAEY LEVSAGDYHL CGLRKPIVGR RKNSNIISSS LVDCWGYNMT RNFVFDKQLH SLSAGSEFNC ALSSKDKSVF CWGDENSSQV ISLIPKEKKF QKIAAGGYHV CGILDGLESR VLCWGKSLEF EEEVTGTSTE EKILDLPPKE PLLAVVGGKF YACGIKRYDH SAVCWGFFVN RSTPAPTGIG FYDLAAGNYF TCGVLTGTSM SPVCWGLGFP ASIPLAVSPG LCIDTPCPPG THELSNQENS PCKFTGSHIC LPCSTSCPPG MYQKSVCTER SDQVCVYNCS SCSSHDCSSN CSSSATSGGK EKGKFWSLQL PIATAEIGFA LFLVAVVSIT AALYIRYRLR NCRCSENDTR SSKDSAFTKD NGKIRPDLDE LQKRRRARVF TYEELEKAAD GFKEESIVGK GSFSCVYKGV LRDGTTVAVK RAIMSSDKQK NSNEFRTELD LLSRLNHAHL LSLLGYCEEC GERLLVYEFM AHGSLHNHLH GKNKALKEQL DWVKRVTIAV QAARGIEYLH GYACPPVIHR DIKSSNILID EEHNARVADF GLSLLGPVDS GSPLAELPAG TLGYLDPEYY RLHYLTTKSD VYSFGVLLLE ILSGRKAIDM HYEEGNIVEW AVPLIKAGDI NALLDPVLKH PSEIEALKRI VSVACKCVRM RGKDRPSMDK VTTALERALA QLMGNPSSEQ PILPTEVVLG SSRMHKKSWR IGSKRSGSEN TEFRGGSWIT FPSVTSSQRR KSSASEGDVA EEEDEGRKQQ EALRSLEEEI GPASPGQSLF LHHNF